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Related Experiment Video

Updated: Jun 16, 2026

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
07:01

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

Advancing three-dimensional tendon imaging using laboratory X-ray phase contrast techniques and refined sample

Charlotte J Maughan Jones1,2, Jayesh Dudhia3, Alberto Astolfo4

  • 1Department of Medical Physics and Biomedical Engineering, University College London, London, UK. c.maughanjones@ucl.ac.uk.

Scientific Reports
|June 13, 2026
PubMed
Summary

Related Concept Videos

X-ray Imaging01:24

X-ray Imaging

German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with X-rays, and by 1900, X-ray was widely...

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medRxiv : the preprint server for health sciences·2025

This study optimized X-ray phase contrast imaging (XPCi) for 3D tendon imaging. Dehydration in ethanol maximized contrast, crucial for understanding tendinopathy anatomy and disease.

Area of Science:

  • Biomedical Imaging
  • Tendon Anatomy
  • Materials Science

Background:

  • Tendinopathy poses significant socio-economic challenges, impacting both athletes and non-athletes.
  • Limited understanding of 3D tendon anatomy hinders diagnosis and treatment of tendinopathy.
  • Traditional histology for pre-clinical tendon imaging is prone to artefacts.

Purpose of the Study:

  • To validate laboratory-based X-ray phase contrast imaging (XPCi) for 3D tendon imaging.
  • To optimize sample preparation protocols for enhanced tendon visualization.
  • To investigate the impact of fixation and dehydration on XPCi contrast.

Main Methods:

  • Equine superficial digital flexor tendons were used as samples.
  • Various fixation (PBS, formalin) and dehydration (ethanol) protocols were tested.
Keywords:
Edge illuminationTendonX-ray phase contrast

Related Experiment Videos

Last Updated: Jun 16, 2026

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
07:01

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

  • Laboratory-based edge illumination XPCi was employed with a custom 3D printed container.

    • Main Results:

    • Ethanol dehydration significantly maximized contrast in tendon tissue.
    • The choice of fixation medium had no discernible effect on XPCi contrast.
    • Successful 3D imaging of tendon volumes was achieved using laboratory-based XPCi.

    Conclusions:

    • Laboratory-based XPCi is a viable method for 3D tendon imaging.
    • Optimized dehydration protocols enhance image quality for tendinopathy research.
    • This technique offers a promising alternative to traditional histology for tendon analysis.