Lipidome and Targeted Metabolome Analysis of Locational Picrorhiza Kurroa Royle. Ex Benth., Using UPLC-PDA and...

Area of Science:

• * Lipidomics and metabolomics
• * Phytochemistry and ethnopharmacology

Background:

• * Lipids are vital biomolecules for cellular structure and signaling.
• * Lipidomics provides advanced understanding of biological lipids.
• * Picrorhiza kurroa is a significant medicinal plant.

Purpose of the Study:

• * To investigate the lipid diversity in Picrorhiza kurroa from three distinct locations in Himachal Pradesh, India.
• * To analyze lipid profiles in leaves, rhizomes, and roots.
• * To understand the impact of location on lipid composition and specialized metabolites.

Main Methods:

• * Ultra-High-Performance Liquid Chromatography-Quadrupole Time-of-Flight-Ion Mobility Spectrometry (UHPLC-QTOF-IMS) for comprehensive lipid profiling.
• * Ultra-Performance Liquid Chromatography-Photodiode Array (UPLC-PDA) for targeted metabolite analysis.
• * Multivariate statistical analyses (OPLS-DA, PCA) to assess sample similarity and variability.

Main Results:

• * Comprehensive lipidome of P. kurroa identified, including Cer, FA, (L)PE, TG, PA, PI, PS, MGDG, and DGDG.
• * Leaves from the Pangi region exhibited higher overall lipid content.
• * Rhizomes from the Pangi region showed elevated levels of picrosides.

Conclusions:

• * Leaves of P. kurroa from the Pangi region are rich in lipids.
• * The study identified key lipids such as Cer, FA, (L)PE, TG, DG, PG, PA, PI, PS, MGDG, and DGDG.
• * Significant variations in lipid and picroside content were observed across different locations and plant parts, highlighting chemotypic diversity.