Related Experiment Video

Updated: Jun 16, 2026

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
10:10

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans

Published on: February 16, 2017

Age-Dependent Effects of Adrenomedullin on Muscle Fiber Composition, Angiogenesis, and Muscle Satellite Cells

Ryota Iyama1, Eriko Kurogi2, Takumi Yokokawa3

  • 1Laboratory of Molecular Adaptations to Exercise, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto, Japan.

Geriatrics & Gerontology International
|June 14, 2026
PubMed
Abstract

Insights

Adrenomedullin (AM) shows age-dependent effects on skeletal muscle. In aged mice, AM improved vascularization and muscle stem cells, enhancing motor function without changing muscle mass.

Area of Science:

  • Muscle physiology
  • Aging research
  • Endocrinology

Background:

  • Sarcopenia is a growing public health concern.
  • Adrenomedullin (AM) has known physiological effects, but its role in skeletal muscle aging is not well understood.
  • This study explores AM's potential to counteract age-related muscle decline.

Purpose of the Study:

  • To investigate the effects of Adrenomedullin (AM) on sarcopenic changes in aged mice.
  • To compare AM's impact on skeletal muscle in young versus aged mice.

Main Methods:

  • Young and aged male mice received subcutaneous Adrenomedullin (AM) or vehicle for 3 months.
  • Multi-muscle analyses included western blotting and immunohistochemistry.
  • Evaluated muscle mass, fiber-type composition, vascularization, and satellite cell populations.

Main Results:

  • AM treatment increased AM levels in plasma and muscle.
  • In aged mice, AM enhanced capillary density and muscle satellite cells (MuSCs), improving vascular and stem cell niches.
  • Aged mice showed improved motor function with AM treatment, despite no change in muscle mass.

Conclusions:

  • Adrenomedullin (AM) exhibits age-dependent effects on skeletal muscle.
  • AM may promote muscle adaptation via vascular remodeling and fiber-type modulation in an age-specific manner.
  • AM presents a potential therapeutic avenue for age-related muscle dysfunction.
Keywords:
adrenomedullinangiogenesismuscle fiber compositionmuscle satellite cellssarcopenia

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