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Related Concept Videos

iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

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Related Experiment Video

Updated: Jun 16, 2026

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids
10:51

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids

Published on: October 13, 2021

Spheroid construction using iPS cell-derived mesenchymal stem and endothelial cells.

Junyi Wang1, Yufan Wu1, Kengo Iwasaki2

  • 1Department of Biomaterials, Osaka Dental University.

Dental Materials Journal
|June 14, 2026
PubMed
Summary
This summary is machine-generated.

We developed a novel method using induced pluripotent stem cells (iPSCs) to create hybrid cell spheroids for bone repair. This feeder-free system combines mesenchymal stem cells (MSCs) and endothelial cells (ECs) for regenerative medicine applications.

Keywords:
Co-cultureSpheroidsTissue engineeringiPSC-derived MSCs and ECs

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Related Experiment Videos

Last Updated: Jun 16, 2026

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids
10:51

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids

Published on: October 13, 2021

Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films
04:14

Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films

Published on: June 19, 2019

Generating Spheroids from Various Chondrocytes using Low-Adhesive Conditions under Gravity and Homemade Mini-Bioreactors
09:03

Generating Spheroids from Various Chondrocytes using Low-Adhesive Conditions under Gravity and Homemade Mini-Bioreactors

Published on: January 31, 2025

Area of Science:

  • Regenerative Medicine
  • Stem Cell Biology
  • Biomaterials Engineering

Background:

  • Induced pluripotent stem cells (iPSCs) offer significant potential for regenerative medicine due to their ability to differentiate into multiple cell types.
  • Developing efficient and scalable methods for generating functional cell constructs is crucial for advancing regenerative therapies.
  • Current methods often involve complex culturing conditions or animal-derived components, posing challenges for clinical translation.

Purpose of the Study:

  • To establish a feeder-free, xeno-reduced workflow for generating uniform hybrid spheroids from iPSC-derived mesenchymal stem cells (MSCs) and endothelial cells (ECs).
  • To investigate the self-assembly and spatial organization of these hybrid spheroids for potential applications in bone repair.
  • To provide a reproducible platform for future studies on vascular maturation within these engineered tissues.

Main Methods:

  • iPSCs were differentiated into MSCs (characterized by CD73+, CD90+, CD105+ expression and multilineage potential) and ECs (CD31+ expression, tube formation assay).
  • MSCs and ECs were combined at a 5:1 ratio and seeded into low-adhesion microwells for spheroid self-assembly under serum-free conditions.
  • Spheroids were analyzed using advanced imaging techniques, including CUBIC clearing and confocal immunofluorescence, to assess cell distribution and structure.

Main Results:

  • Uniform, size-controlled hybrid spheroids composed of iPSC-derived MSCs and ECs were successfully generated within 4 hours.
  • Confocal immunofluorescence confirmed the distinct spatial organization of CD90+ (MSC) and CD31+ (EC) cells within the 3D spheroid structure.
  • While both cell types coexisted, evidence of lumen formation was not observed at this initial stage, indicating potential for further maturation.

Conclusions:

  • A reproducible, feeder-free, and xeno-reduced system was established for creating hybrid spheroids from a single iPSC source.
  • The generated spheroids demonstrate the potential for controlled self-assembly and spatial arrangement of different cell types.
  • This platform serves as a foundation for future research into vascularization strategies and therapeutic applications in bone regeneration.