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Updated: Jun 16, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
A summary guide for detecting and reducing nitrous oxide infrastructure leaks in healthcare facilities
Eugenie A Kayak1, Rebecca E McIntyre1,2, Forbes McGain1,3
1Department of Critical Care, University of Melbourne, Melbourne, Australia.
Abstract:
Leaks from nitrous oxide (N2O) infrastructure have been identified as a significant contributor to the greenhouse gas emissions of healthcare facilities. Recent studies in Australia and in the United Kingdom have found at least half (and often more than 70%) of the N2O supplied to many healthcare facilities leaks from medical pipelines and associated infrastructure before clinical administration. To assist in addressing this issue, the University of Melbourne, in collaboration with the Interim Australian Centre for Disease Control and the Australian Government's Department of Health and Aged Care, published Detecting and reducing nitrous oxide leaks in healthcare facilities - a practical guide (the 'Guide') in 2024. This article summarises the Guide and best practice approaches for detection and reduction of N2O leaks in healthcare facilities across Australia. It is intended to assist clinicians and facility managers to identify appropriate methods to test for N2O leaks, make an informed choice about the most appropriate way to supply N2O to a facility and reduce waste from N2O leaks in their healthcare facility.
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