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Updated: Jun 16, 2026

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
Published on: December 19, 2018
PDIA1 promotes androgen receptor activation and prostate cancer cell survival through enhancing HMMR stability
Sishu Yu1,2, Zijian Kuang1,2, Ping Yao1,2
1School of Biology and Biological Engineering, South China University of Technology, University Town, Guangzhou, China.
Abstract:
To sustain rapid proliferation, cancer cells increase protein synthesis, intensifying reliance on protein disulfide isomerase A1 (PDIA1). It is largely unknown whether disulfide bond formation of PDIA1 substrates is driven by one or both CGHC motifs. Using active-site trapping mutants in prostate cancer cells combined with mass spectrometry, we identified 29 proteins uniquely bound to the C53GHC56 domain and 20 proteins uniquely bound to the C397GHC400 domain. Hyaluronan-mediated motility receptor (HMMR) was validated as a PDIA1 C397GHC400-specific substrate, with PDIA1 catalysing disulfide bond formation between Cys242 and Cys293. PDIA1 knockdown induced HMMR ubiquitination, blocked androgen receptor nuclear translocation, and suppressed prostate cancer cell growth, survival, and migration. These findings reveal a previously unknown role of PDIA1 in prostate cancer biology.
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