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Updated: Jun 16, 2026

Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology

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Published on: June 25, 2014

Clarifying Frizzled 2 function in development through genetically validated mouse models.

Megan N Michalski¹, Cassandra R Diegel¹, Zhendong A Zhong¹

¹Department of Cell Biology, Van Andel Institute, Grand Rapids, Michigan 49503, USA.

Disease Models & Mechanisms

|June 15, 2026

Summary

This summary is machine-generated.

Frizzled-2 (Fzd2) is crucial for limb development, not just redundant. A new mouse model shows Fzd2 knockout causes limb shortening, clarifying its essential role in embryonic development.

Keywords:

CRISPR/Cas9 Chondrocytes Cre-lox recombination Frizzled Fzd Homologous recombination Limb development Whole-genome sequencing Wnt signaling

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Area of Science:

Developmental Biology
Genetics
Molecular Biology

Background:

Frizzled (Fzd) receptors, particularly Fzd2, are thought to have functional redundancy.
Previous studies on Fzd2 global knockout mice yielded conflicting results regarding embryonic lethality and Fzd2's specific role.
Existing conditional Fzd2 knockout models were found to be ineffective in fully eliminating Fzd2 expression.

Purpose of the Study:

To generate and validate a new conditional mouse model for Fzd2.
To investigate the specific role of Fzd2 in embryonic development, particularly in limb formation.
To resolve discrepancies in previous Fzd2 knockout studies.

Main Methods:

Generation of a novel conditional Fzd2 knockout mouse model by flanking the Fzd2 gene with loxP sites.
Confirmation of Cre-mediated recombination leading to a Fzd2 null allele.
Analysis of phenotypes resulting from global and limb-specific Fzd2 deletion.

Main Results:

Global deletion of Fzd2 in the new model did not result in embryonic lethality.
Limb-specific deletion of Fzd2 led to significant limb shortening.
The new conditional model effectively allows for tissue-specific Fzd2 ablation.

Conclusions:

Fzd2 plays a critical, non-redundant role in mammalian limb development.
The validated conditional model is essential for future tissue-specific Fzd2 research.
Thorough validation of mouse models is crucial for accurate scientific findings.