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Related Experiment Video

Updated: Jun 16, 2026

A Comprehensive Protocol for Manual Segmentation of the Medial Temporal Lobe Structures
12:30

A Comprehensive Protocol for Manual Segmentation of the Medial Temporal Lobe Structures

Published on: July 2, 2014

Evaluation of a New Paracingulate Sulcus Identification and Measurement Protocol.

Héloïse de Vareilles1, Samantha C Mitchell2, Jane R Garrison2

  • 1Department of Psychiatry, University of Cambridge, Cambridge, UK.

Human Brain Mapping
|June 15, 2026
PubMed
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A new protocol improves identification of the paracingulate sulcus (PCS) by incorporating the intra-limbic sulcus and 3D visualization. This enhances reliability in measuring PCS length, aiding research on brain folding patterns.

Area of Science:

  • Neuroscience
  • Neuroanatomy
  • Brain Imaging

Background:

  • The anterior cingulate cortex exhibits variable folding patterns.
  • The paracingulate sulcus (PCS) is challenging to identify due to this variability.

Purpose of the Study:

  • To assess the benefits of a novel protocol for identifying and measuring the paracingulate sulcus (PCS).
  • To compare the new protocol with the previous standard using 3D brain reconstructions.

Main Methods:

  • Applied both old and new protocols to identify the PCS in 50 UK subjects.
  • Utilized BrainVISA software for 3D reconstructions and automatic PCS length computation.
  • Assessed inter-rater reliability and correlation of PCS length measurements between protocols.

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Preparation of Parasagittal Slices for the Investigation of Dorsal-ventral Organization of the Rodent Medial Entorhinal Cortex
09:45

Preparation of Parasagittal Slices for the Investigation of Dorsal-ventral Organization of the Rodent Medial Entorhinal Cortex

Published on: March 28, 2012

Related Experiment Videos

Last Updated: Jun 16, 2026

A Comprehensive Protocol for Manual Segmentation of the Medial Temporal Lobe Structures
12:30

A Comprehensive Protocol for Manual Segmentation of the Medial Temporal Lobe Structures

Published on: July 2, 2014

Preparation of Parasagittal Slices for the Investigation of Dorsal-ventral Organization of the Rodent Medial Entorhinal Cortex
09:45

Preparation of Parasagittal Slices for the Investigation of Dorsal-ventral Organization of the Rodent Medial Entorhinal Cortex

Published on: March 28, 2012

Main Results:

  • The new protocol demonstrated improved accuracy by correctly distinguishing the PCS from the intra-limbic sulcus.
  • Intra-class correlations for PCS length were high (0.85-0.86) with the new protocol, compared to 0.81 for the old protocol.
  • High correlation (0.85) in PCS length was achieved when excluding disagreements between protocols.

Conclusions:

  • Incorporating intra-limbic sulcus identification and 3D visualization enhances reliable PCS identification.
  • The new protocol is a reliable tool for research on cingulate and paracingulate cortical folding.