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Comparative In Vitro Study on the Demineralization Potential of Different Milk Types on Primary Teeth
Amulya Jalumuru1, Sri R Maganti1, Punithavathy Naren2
1Department of Pedodontics and Preventive Dentistry, Lenora Institute of Dental Sciences, Rajamahendravaram, Andhra Pradesh, India.
International Journal of Clinical Pediatric Dentistry
|June 15, 2026
Summary
Human breast milk and bovine milk protect infant teeth, while soy milk and infant formula can harm enamel. This study compared the dental effects of different milk types on primary teeth.
Area of Science:
- Pediatric Dentistry
- Dental Materials Science
- Biochemistry
Background:
- Milk is crucial for infant nutrition and has potential roles in enamel health.
- Lactose in milk can be fermented, potentially leading to caries under certain conditions.
- Understanding milk's impact on dental health is vital due to widespread consumption by children.
Purpose of the Study:
- To evaluate the influence of different milk types on primary teeth's dental health.
- To compare the demineralization potential of human breast milk, bovine milk, infant formula, and soy milk.
- To assess the effects on enamel mineral content, pH, and microhardness.
Main Methods:
- 40 primary incisors were analyzed for initial mineral content and pH.
- Teeth were immersed in four different milk types: human breast milk, bovine milk, infant formula, and soy milk.
- Post-immersion analysis included mineral content, pH, and microhardness testing.
Main Results:
- Calcium content increased in all milk types, with the highest gain observed in breast milk.
- Significant pH drops and reduced hardness were noted with infant formula and soy milk.
- Infant formula also led to mineral loss from the enamel.
Conclusions:
- Human breast milk and bovine milk demonstrated a protective effect on enamel.
- These protective effects were evidenced by increased calcium, stable pH, and higher microhardness.
- Soy milk and infant formula showed demineralizing potential, negatively impacting enamel integrity.