Related Experiment Video
Updated: Jun 27, 2026
05:28
Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis
Published on: December 9, 2022
Machine learning identifies PPARG as a diagnostic biomarker for sepsis linked to CD14/NF-κB signaling: integrated
Yingying Ji1, Xin Xiao1, Yanou Li1
1Department of Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, China.
Frontiers in Cellular and Infection Microbiology
|June 15, 2026
Summary
This study identified PPARG as a promising blood biomarker for sepsis diagnosis, linked to the CD14/NF-κB immune pathway. Findings support developing host-response biomarkers for sepsis and understanding PPARG
Area of Science:
- Immunology and Infectious Diseases
- Genomics and Bioinformatics
- Biomarker Discovery
Background:
- Sepsis diagnosis is challenging due to heterogeneity; early detection is critical.
- CD14 is key in innate immunity, but its downstream regulatory network is unclear.
- Identifying CD14-associated biomarkers can improve sepsis diagnostics.
Purpose of the Study:
- To identify CD14-associated blood-based diagnostic biomarkers for sepsis.
- To explore the regulatory mechanisms linking CD14 to sepsis immunometabolism.
Main Methods:
- Analyzed whole-blood transcriptomic data from discovery (GSE236713) and validation (GSE65682) cohorts.
- Utilized machine learning (LASSO, random forest, ANN) and WGCNA to identify candidate genes.
- Performed in vitro validation in macrophages using CD14 manipulation and NF-κB inhibition.
Main Results:
- Identified five feature genes, including PPARG, MMP9, C1QC, MS4A4A, and ARG1.
- An artificial neural network (ANN) classifier showed high diagnostic performance (AUC=0.974 internal, 0.953 external).
- PPARG demonstrated strong diagnostic ability (AUC=0.994), correlated with SOFA score, and predicted ICU outcomes.
Conclusions:
- PPARG is a potential diagnostic biomarker for sepsis, associated with CD14/NF-κB signaling.
- Findings support developing host-response-based diagnostic signatures for sepsis.
- Further research into PPARG's role in sepsis immunometabolism is warranted.
