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Related Concept Videos

Endoscopic Procedures II: Colonoscopy01:25

Endoscopic Procedures II: Colonoscopy

The colon, or large intestine, is the final segment of the digestive system. Its primary functions include absorbing water and vitamins produced by gut bacteria and transforming waste from liquid to solid to form stool. In adults, the large intestine is approximately 5 feet long and consists of four main sections:
Imaging Studies III: Gastrointestinal Motility Studies and Virtual Colonoscopy01:26

Imaging Studies III: Gastrointestinal Motility Studies and Virtual Colonoscopy

This lesson explores three gastrointestinal imaging techniques: radionuclide testing, colonic transit studies, and virtual colonoscopy.
Radionuclide Testing
Radionuclide testing is a sophisticated medical technique for assessing gastrointestinal motility. It focuses on gastric emptying and colonic transit time. Radioactive markers track the movement of food through the digestive system, providing insights into gastrointestinal disorders.
In gastric emptying studies, a meal's liquid and solid...
One-Compartment Open Model for IV Bolus Administration: Estimation of Clearance00:56

One-Compartment Open Model for IV Bolus Administration: Estimation of Clearance

Clearance is a key pharmacokinetic parameter that quantifies the volume of body fluid from which a drug is entirely removed within a specific time frame. It is crucial in assessing how a drug is eliminated from the body and has critical clinical applications.
In the one-compartment open model for intravenous (IV) bolus administration, clearance is estimated by dividing the elimination rate by the plasma drug concentration. This equation leverages the elimination rate constant and the apparent...
Endoscopic Procedures IV: Sigmoidoscopy and Laproscopy01:26

Endoscopic Procedures IV: Sigmoidoscopy and Laproscopy

Sigmoidoscopy and laparoscopy are distinct medical procedures that enable physicians to internally inspect different parts of the GI tract. Although they serve different purposes, each is essential for diagnosing and, in some cases, treating various medical conditions.
Sigmoidoscopy
Sigmoidoscopy is a diagnostic procedure that uses a flexible sigmoidoscope equipped with a light source and camera to examine the rectum and sigmoid colon. The procedure involves inserting the tube through the anus...
Endoscopic Studies I: Bronchoscopy and Thoracoscopy01:30

Endoscopic Studies I: Bronchoscopy and Thoracoscopy

Endoscopy is a non-surgical medical technique used to examine a person's internal organs and vessels. This lesson will focus on two types of endoscopic studies: bronchoscopy and thoracoscopy.
Bronchoscopy
Description
Bronchoscopy is a procedure that involves direct visualization of the larynx, trachea, and bronchi for diagnostic and therapeutic purposes. A flexible fiber optic or rigid bronchoscope is used to carry out the procedure. The fiber-optic bronchoscope is more frequently used due to...
Endoscopic Procedures I: Esophagogastroduodenoscopy01:29

Endoscopic Procedures I: Esophagogastroduodenoscopy

An Esophagogastroduodenoscopy (EGD) is a diagnostic procedure in which an endoscopist uses a flexible, lighted endoscope to visualize the upper gastrointestinal (GI) tract. The procedure includes visualizing the oropharynx, esophagus, stomach, and the first part of the small intestine, the duodenum.
During an EGD, the endoscope can be used to:

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Related Experiment Video

Updated: Jun 16, 2026

Structured Approach to Colonoscopy Technique Optimization: A Single-Center Experience with Novice Endoscopists
03:43

Structured Approach to Colonoscopy Technique Optimization: A Single-Center Experience with Novice Endoscopists

Published on: July 11, 2025

Optimization of colonoscope bedside precleaning protocol: an orthogonal experimental study.

Bingru Li1, Jinpeng Jiang1, Yingying Wang1

  • 1Department of Digestive Endoscopy Center, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China.

Frontiers in Cellular and Infection Microbiology
|June 15, 2026
PubMed
Summary

Optimizing colonoscope precleaning with an alkaline solution and 20-second suction significantly reduces contamination. Adenosine triphosphate (ATP) testing effectively screens biopsy channel cleanliness during cleaning, aiding quality control in endoscopy.

Keywords:
bedside precleaningdecontaminationendoscope reprocessing colonoscopeexperimentalorthogonal

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E-Patient Counseling Trial (E-PACO): Computer Based Education versus Nurse Counseling for Patients to Prepare for Colonoscopy
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E-Patient Counseling Trial (E-PACO): Computer Based Education versus Nurse Counseling for Patients to Prepare for Colonoscopy

Published on: August 1, 2019

Related Experiment Videos

Last Updated: Jun 16, 2026

Structured Approach to Colonoscopy Technique Optimization: A Single-Center Experience with Novice Endoscopists
03:43

Structured Approach to Colonoscopy Technique Optimization: A Single-Center Experience with Novice Endoscopists

Published on: July 11, 2025

E-Patient Counseling Trial (E-PACO): Computer Based Education versus Nurse Counseling for Patients to Prepare for Colonoscopy
06:28

E-Patient Counseling Trial (E-PACO): Computer Based Education versus Nurse Counseling for Patients to Prepare for Colonoscopy

Published on: August 1, 2019

Area of Science:

  • Endoscopic reprocessing and infection control.
  • Biomedical engineering and device safety.
  • Microbiology and healthcare-associated infections.

Background:

  • Effective precleaning of colonoscopes is crucial for preventing cross-contamination and ensuring patient safety.
  • Current precleaning protocols lack standardized optimization, potentially impacting the efficacy of subsequent cleaning and disinfection.
  • Adenosine triphosphate (ATP) bioluminescence testing offers a rapid method for assessing surface cleanliness, but its correlation with microbial contamination in colonoscope precleaning requires further evidence.

Purpose of the Study:

  • To optimize the bedside precleaning protocol for colonoscopes using an orthogonal experimental design.
  • To evaluate the correlation between adenosine triphosphate (ATP) bioluminescence testing and microbial culture results in assessing colonoscope cleanliness.
  • To establish an evidence-based foundation for quality control in endoscopic reprocessing.

Main Methods:

  • An L9(3^4) orthogonal experimental design was employed to test nine different bedside precleaning protocols for 180 colonoscopes.
  • Four factors (detergent type, solution replacement frequency, suction duration, and delay time) were evaluated at three levels each.
  • Adenosine triphosphate (ATP) levels and microbial counts were measured post-precleaning, post-cleaning, and post-disinfection. Spearman correlation analysis was used to assess the relationship between ATP and microbial results.

Main Results:

  • Alkaline solution (A3) and 20-second suction (C2) were identified as statistically significant factors (P=0.035 and P=0.048) for reducing ATP values and microbial counts post-precleaning.
  • No tested factors significantly influenced ATP levels or microbial counts after the full cleaning and disinfection process (P>0.05).
  • ATP testing and microbial cultures showed high consistency in evaluating biopsy channel cleanliness during the cleaning phase.

Conclusions:

  • The optimized colonoscope bedside precleaning protocol involves using an alkaline solution, replacing it every four hours, employing 20-second suction, and a delay of ≤3 hours.
  • ATP testing serves as a reliable screening tool for immediate process intervention during endoscope cleaning, correlating well with microbial culture results.
  • While ATP testing is effective for assessing cleanliness during cleaning, its utility is limited after high-level disinfection (HLD).