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Related Experiment Video

Updated: Jun 16, 2026

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Coupling Single Molecules to DNA-Based Optical Antennas with Position and Orientation Control.

Aleksandra K Adamczyk¹, Fangjia Zhu¹, Daniel Schäfer²

¹Department of Physics, University of Fribourg, Fribourg CH-1700, Switzerland.

|June 15, 2026

Summary

This summary is machine-generated.

Researchers used DNA origami to precisely control single-molecule orientation and position relative to optical antennas. This achieved a 5-fold increase in fluorescence intensity, paving the way for advanced nanophotonic devices.

Keywords:

DNA nanotechnology nanophotonics optical antennas plasmonics single-molecule fluorescence single-photon sources

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Area of Science:

Nanophotonics
Molecular Biophysics
Materials Science

Background:

Optical antennas are crucial for controlling light-matter interactions with single-photon emitters.
Emitter-antenna coupling depends on spectral overlap, distance, and orientation, with orientation control being a significant challenge.

Purpose of the Study:

To demonstrate precise control over the orientation and position of single emitters within DNA origami-assembled optical antennas.
To investigate the impact of emitter orientation on fluorescence enhancement.

Main Methods:

Utilized DNA origami for precise assembly of dimer optical antennas.
Positioned single fluorescent molecules at the antenna gap with controlled orientations (parallel/perpendicular).
Correlated fluorescence measurements with scanning electron microscopy (SEM) and numerical simulations.

Main Results:

Achieved a 5-fold higher average fluorescence intensity for emitters aligned parallel to the antenna axis.
Observed a maximum fluorescence enhancement of approximately 1400-fold.
Variations in fluorescence enhancement were attributed to minor changes in emitter orientation and gap size.

Conclusions:

DNA origami provides a versatile platform for full control of emitter-antenna coupling.
This technique enables the development of self-assembled nanophotonic devices with enhanced and uniform performance.
Establishes a pathway for designing optimized nanophotonic devices through precise molecular control.