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Exploring the shared biomarkers between acute ischemia stroke and diabetes by transcriptome sequencing.
Cong Xu1, Yonghong Xu2, Guangming Wang3
1Cong Xu School of Clinical Medicine, Dali University, Dali 671000, Yunnan, People's Republic of China.
Pakistan Journal of Medical Sciences
|June 15, 2026
Summary
This study reveals shared transcriptomic changes in acute ischemic stroke (AIS) and diabetes, identifying hsa-miR-619-5p as a key regulator. This finding offers potential biomarkers and therapeutic targets for managing both conditions.
Area of Science:
- Genomics
- Molecular Biology
- Biomedical Science
Background:
- Acute ischemic stroke (AIS) and diabetes are significant global health concerns.
- Understanding the molecular links between AIS and diabetes is crucial for effective management.
- Transcriptomic analysis offers a powerful approach to uncover shared genetic signatures and molecular mechanisms.
Purpose of the Study:
- To identify shared genetic signatures and molecular mechanisms linking AIS and diabetes.
- To characterize differentially expressed non-coding RNAs (ncRNAs) and messenger RNAs (mRNAs).
- To construct regulatory networks and elucidate cross-talk between AIS and diabetes.
Main Methods:
- Transcriptome sequencing of peripheral blood samples from AIS and diabetes patients.
- Identification of differentially expressed miRNAs, mRNAs, lncRNAs, and circRNAs.
- Functional enrichment analysis (GO, KEGG) and reconstruction of a competing endogenous RNA (ceRNA) network.
Main Results:
- Identified thousands of differentially expressed ncRNAs and mRNAs between AIS and diabetes groups.
- Pathway analysis revealed circRNAs modulating immune signaling and miRNA-target genes in cancer/stem cell pathways.
- Constructed a ceRNA network with hsa-miR-619-5p as a central hub coordinating interactions.
Conclusions:
- Convergent transcriptomic alterations link AIS and diabetes comorbidity.
- hsa-miR-619-5p acts as a hub regulator of immune-metabolic crosstalk.
- The ceRNA network provides a framework for understanding ncRNA roles in inflammation and suggests potential biomarkers and therapeutic targets.
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