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Exogenous Kisspeptin-10 inhibits ovarian cancer progression through targeting the SP1-hTERT-ZEB1 regulatory axis

Hetvi Shah1, Abin Thomas2, Parth Pandya1

  • 1TREE Lab, Division of Biomedical and Life Sciences, School of Science, Navrachana University, Vadodara, Gujarat India.

Cytotechnology
|June 15, 2026
PubMed

Insights

Kisspeptin-10 (KP-10) effectively inhibits ovarian cancer cell growth and metastasis by targeting the SP1-ZEB1-hTERT pathway. This study reveals KP-10

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Ovarian cancer is a leading cause of gynecological cancer deaths, characterized by high metastatic potential and telomerase reactivation.
  • The SP1-ZEB1-hTERT pathway plays a crucial role in ovarian cancer progression and metastasis.

Purpose of the Study:

  • To investigate the effects of exogenous Kisspeptin-10 (KP-10) on the SP1-ZEB1-hTERT pathway in SKOV-3 ovarian cancer cells.
  • To elucidate the molecular mechanisms underlying KP-10's anti-cancer activity.

Main Methods:

  • In vitro experiments using SKOV-3 ovarian cancer cell lines.
  • Computational techniques including bioinformatics analysis of the KISS1 promoter.
  • Assays to measure cell proliferation, migration, invasion, and gene/microRNA expression.

Main Results:

  • KP-10 significantly inhibited SKOV-3 cell proliferation, migration, and invasion in a dose-dependent manner (IC₅₀ = 72.28 nM).
  • KP-10 modulated the expression of key transcriptional factors (SP1, CDX2, GATA2, FLI1, ZEB1) and hTERT, indicating telomerase pathway interference.
  • KP-10 influenced tumor-suppressive microRNAs (miR-200, miR-345, miR-577), known inhibitors of epithelial-mesenchymal transition and cancer development.

Conclusions:

  • KP-10 effectively suppresses ovarian cancer cell proliferation and metastasis.
  • KP-10 exerts its anti-cancer effects by modulating the SP1-ZEB1-hTERT pathway and associated transcriptional factors and microRNAs.
  • These findings highlight KP-10 as a potential therapeutic agent for ovarian cancer.
Keywords:
Kisspeptin-10, Ovarian Cancer, Metastasis, Transcription Regulation, Epithelial-Mesenchymal Transition

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