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Related Concept Videos

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Related Experiment Video

Updated: Jun 16, 2026

Improving CRISPR-Cas9 Screens in CAR T Cells: A Refined Method for Library Preparation
08:03

Improving CRISPR-Cas9 Screens in CAR T Cells: A Refined Method for Library Preparation

Published on: January 2, 2026

CRISPR-screen informed engineered T cell therapies.

Karrie Wong1, Conor Calnan1, Micah J Benson1

  • 1KSQ Therapeutics, Lexington, MA, United States.

Frontiers in Immunology
|June 15, 2026
PubMed
Summary
This summary is machine-generated.

CRISPR screens identify targets to enhance T cell therapies for solid tumors. This approach overcomes the immunosuppressive tumor microenvironment and antigen scarcity, improving adoptive T cell therapy efficacy.

Keywords:
CRISPRT cellTILadoptive cell therapysolid tumor

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Area of Science:

  • Immunology
  • Genetics
  • Oncology

Background:

  • Adoptive T cell therapies show promise for B cell malignancies but face challenges in solid tumors.
  • Solid tumors present barriers like immunosuppressive microenvironments and limited target antigens.
  • CRISPR screens offer a method to map genes influencing T cell anti-tumor activity.

Purpose of the Study:

  • To review CRISPR knockout screen insights for T cell anti-tumor function.
  • To identify convergent targets and pathways for enhancing T cell therapies.
  • To propose a framework for CRISPR screen-enabled target prioritization in solid tumors.

Main Methods:

  • Pooled CRISPR knockout screens in T cells to identify functional targets.
  • Review of recent insights from CRISPR screens in the context of the tumor microenvironment (TME).
  • Application of principles to enhance Tumor Infiltrating Lymphocyte (TIL) therapy.

Main Results:

  • CRISPR screens reveal key targets and pathways regulating T cell function within the TME.
  • Identification of strategies to overcome tumor-intrinsic immunosuppressive barriers.
  • Development of a framework for prioritizing targets to improve T cell therapies.

Conclusions:

  • CRISPR screens are crucial for understanding and enhancing T cell therapies against solid tumors.
  • Targeting TME barriers and antigen recognition is key for therapeutic success.
  • This approach can optimize engineered T cell therapies like TIL therapy for personalized cancer treatment.