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Related Experiment Video

Updated: Jun 16, 2026

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

CISP, an Intrinsically Disordered Cold-Inducible Barley Protein, Functions as a Small RNA Chaperone.

Yutaro Okumura¹, Md Maksudul Haque¹, Shin-Ichiro Kidou^1,2

¹Graduate School of Science Nagoya City University Nagoya Japan.

|June 15, 2026

Summary

This summary is machine-generated.

A novel barley protein, CISP, enhances growth in low temperatures by acting as an RNA chaperone. This discovery offers new strategies for improving crop cold tolerance through molecular breeding.

Keywords:

CISP RNA chaperone barley cold tolerance intrinsically disordered protein (IDPs)

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Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions

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Published on: June 7, 2020

Area of Science:

Plant Biology
Molecular Biology
Biochemistry

Background:

Low temperatures pose significant stress, limiting plant growth and crop yields.
Understanding molecular mechanisms of cold tolerance is crucial for agricultural improvement via molecular breeding.

Purpose of the Study:

To investigate the function of CISP, a barley protein induced by cold stress.
To determine CISP's role in promoting growth under low temperatures and its molecular activity.

Main Methods:

Heterologous expression of CISP in *Escherichia coli* and *Arabidopsis thaliana* to assess growth promotion.
RNA chaperone assays using RNA beacons to evaluate CISP's effect on RNA secondary structures.

Main Results:

CISP expression enhanced bacterial and plant seedling growth at low temperatures.
CISP demonstrated RNA chaperone activity by reducing and destabilizing RNA secondary structures.
CISP, a basic protein with an intrinsically disordered N-terminal region, lacks canonical RNA-binding domains.

Conclusions:

CISP promotes growth in heterologous organisms under cold stress, suggesting a conserved function.
CISP exhibits RNA chaperone activity, potentially representing a novel class of RNA chaperones.
CISP offers new insights into barley cold tolerance mechanisms and potential applications in crop improvement.