The dose-dependent anesthetic effect of ciprofol on 8-week-old C57BL/6J mice

The research is to explore the half effective dose (ED₅₀) and 95% effective dose (ED₉₅) of ciprofol with intraperitoneal injection of 8-week-old C57BL/6J adult mice, which will provide experimental basis for the reasonable dose design of ciprofol in preclinical basic research.

C57BL/6J mice were selected and divided into 8 dose groups (50, 55, 60, 65, 70, 80, 90, 100 mg/kg), with 12 males and 12 females in each group. The ciprofol was administered by intraperitoneal injection, and the latency of loss of righting reflex (LORR) and recovery time of righting reflex (RORR) were recorded. Probit regression analysis was used to calculate the ED₅₀ and ED₉₅ values of ciprofol anesthesia in mice.

There was a significant positive correlation between the dose of ciprofol and the positive rate of anesthesia in mice, and the recovery time of anesthesia was prolonged with the increase of dose. The ED₅₀ of ciprofol in anesthesia was 56.014 mg/kg for male and 54.783 mg/kg for female, and the ED95 was 91.622 mg/kg for male and 82.212 mg/kg for female. There was no significant difference in the anesthetic dose between male and female mice.

In this study, the ED₅₀ and ED₉₅ of ciprofol in male and female mice was determined. Our results demonstrate that ciprofol induces a dose-dependent anesthetic effect in mice, but gender has no significant impact on its efficacy. Collectively, these data provide a critical dosimetric basis for the standardized application of ciprofol in preclinical research.