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Updated: Jun 16, 2026

In vitro Functional Characterization of Mouse Colorectal Afferent Endings
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Functional characterization and classification of mechanosensitive bladder afferents.

Guadalupe Manrique-Maldonado1, Xuejiao Sun1, Stephanie L Daugherty2

  • 1Renal-Electrolyte Division, Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15213, USA.

Biorxiv : the Preprint Server for Biology
|June 15, 2026
PubMed
Summary

Researchers classified mechanosensitive bladder afferents into three distinct types based on their responses to physiological and noxious bladder filling. This classification advances understanding of bladder sensory neuron function.

Keywords:
PIEZO channelsYoda1afferent activitysensory neuronsurinary bladder

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Area of Science:

  • Neuroscience
  • Urology
  • Physiology

Background:

  • Normal bladder function depends on sensory neurons detecting mechanical and chemical signals.
  • The specific types and functions of these sensory neurons are not fully understood.

Purpose of the Study:

  • To develop a framework for classifying mechanosensitive bladder afferents.
  • To identify distinct functional types of bladder afferent neurons.

Main Methods:

  • Classifying afferents based on responses to physiological (15 μl/min) and noxious filling (high pressures).
  • Analyzing firing rates during varying filling speeds and pressures.
  • Utilizing PIEZO1 channel activator (Yoda1) to assess afferent responses.

Main Results:

  • Identified three distinct mechanosensitive bladder afferent types: Type I (linear response), Type II (plateau response) to physiological filling, and Type III (noxious stimulation response).
  • Fast filling increased discharge in all types, most significantly in Type III.
  • Yoda1 modulated firing rates, supporting distinct activation mechanisms for each type.

Conclusions:

  • Established a reliable method for classifying bladder afferents.
  • Provided evidence for three functionally distinct mechanosensitive bladder afferent populations.
  • These populations are regulated by unique mechanisms, advancing bladder sensory research.