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Updated: Jun 16, 2026
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Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate
Published on: October 31, 2012
The Sr-HA-loaded PLGA cage structure combines cells to construct a bone tissue repair unit
Huixing Yi1, Guowen Duan1,2, Siyu Li2
1School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 610031, China.
Regenerative Biomaterials
|June 15, 2026
Summary
This study presents a novel injectable bone repair unit using multicellular microspheres. The innovative scaffold promotes osteoporotic bone healing by combining cells and strontium ions for enhanced bone formation and reduced resorption.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Orthopedic Research
Background:
- Osteoporosis leads to reduced bone mass and increased fracture risk.
- Fracture repair in osteoporotic bone is challenging due to compromised bone quality and risk of secondary injury.
Purpose of the Study:
- To develop an injectable bone repair unit for osteoporotic bone defects.
- To leverage the synergistic effects of cells and strontium ions for enhanced bone regeneration.
Main Methods:
- Fabrication of cage-structured PLGA microspheres (PLGA-CAS).
- Coating PLGA-CAS with strontium-doped hydroxyapatite (Sr-HA) particles to form SrHP.
- Culturing pre-osteoblasts (MC3T3-E1) and endothelial cells (HUVECs) on SrHP to create multicellular microspheres.
- Evaluating the blended microsphere system (MH-SrHP) for osteogenic differentiation, angiogenesis, and osteoclast inhibition.
Main Results:
- SrHP microspheres exhibited a porous structure with anchored Sr-HA particles.
- Microspheres loaded with MC3T3-E1 (M-SrHP) and HUVECs (H-SrHP) showed high cell viability and injectability.
- The blended MH-SrHP system promoted osteogenic differentiation, angiogenesis, and inhibited osteoclast differentiation in vitro.
Conclusions:
- The developed multifunctional construct serves as a streamlined platform for osteoporotic bone repair.
- This innovative therapeutic strategy shows promise for clinical translation in treating osteoporotic bone defects.
