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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Multi-kingdom gut microbiota analysis identifies bacterial-viral association in multiple myeloma
Lu Liu1, Jingmei Liu1, Jianxia He1
1Department of Hematology, Xu Chang Central Hospital, Xu Chang, Henan, China.
Frontiers in Microbiology
|June 15, 2026
Summary
Multiple myeloma (MM) is linked to gut microbiome changes, including bacteria and viruses. This study found distinct viral and bacterial signatures in MM patients, suggesting a role for the virome in disease progression.
Area of Science:
- Microbiology
- Virology
- Oncology
Background:
- Gut microbiome alterations are associated with multiple myeloma (MM) progression.
- Previous research focused on bacteria, neglecting the role of the virome (viruses) in MM.
Purpose of the Study:
- To investigate the interplay between the gut microbiota (bacteria and viruses) and multiple myeloma (MM) development.
- To identify specific microbial and viral signatures associated with MM.
Main Methods:
- Shotgun metagenomic sequencing of fecal samples from 28 MM patients and 20 healthy controls.
- Taxonomic profiling of bacterial and viral communities using Kraken2 classifier.
Main Results:
- MM patients exhibited microbial dysbiosis with altered bacterial phyla (increased Pseudomonadota, decreased Bacillota).
- Enrichment of specific viral taxa (Heunggongvirae, Uroviricota, Punavirus) was observed in MM patients.
- Functional analysis revealed altered metabolic pathways, including reduced amino acid/bile acid biosynthesis and increased biofilm formation/CAMP resistance.
Conclusions:
- Distinct bacterial and viral signatures are associated with multiple myeloma (MM).
- These findings highlight the importance of the gut virome in MM pathogenesis and warrant further investigation.
Keywords:
bacterial-viral associationgut microbiotagut-brain axismetagenome sequencingmultiple myelomaMore Related Videos
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