Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aspirin Dosing Frequency and Dose Influence Thromboxane Suppression Despite Uniform Inhibition of Arachidonic Acid-induced Platelet Aggregation.

TH open : companion journal to thrombosis and haemostasis·2026
Same author

Poly(sulfobetaine-<i>co</i>-oligoethylene Glycol Methyl Ether Methacrylate) Copolymers with Improved Anti-Fouling and Anti-Coagulant Properties.

Biomacromolecules·2026
Same author

Prior anticoagulation experience and bleeding risk with the factor XI inhibitor abelacimab in AZALEA-TIMI 71.

Blood·2026
Same author

Long-Term Hydrophilic, Anti-Clotting, and Anti-Fibrotic Dynamic Covalent Silicone-Based Biomaterials.

Advanced healthcare materials·2026
Same author

Pharmacologic Factor XI/XIa Inhibitors: What the Laboratory Hematologist Needs to Know.

Clinics in laboratory medicine·2026
Same author

Canagliflozin is a novel androgen receptor pathway inhibitor for castrate-sensitive and castrate-resistant prostate cancer.

Cancer letters·2026
Same journal

Heparin to prevent recurrent placenta-mediated pregnancy complications in women with antiphospholipid syndrome: a systematic review.

Research and practice in thrombosis and haemostasis·2026
Same journal

Thromboembolic ischemic stroke induces neutrophil recruitment and matrix metalloproteinase-9 release to enhance ischemic brain injury.

Research and practice in thrombosis and haemostasis·2026
Same journal

Real-world investigation of platelet apoptosis for mechanistic insights into severe immune thrombocytopenia and bleeding.

Research and practice in thrombosis and haemostasis·2026
Same journal

Antiphospholipid antibody formation during immune checkpoint inhibition in patients with cancer: a prospective cohort study.

Research and practice in thrombosis and haemostasis·2026
Same journal

Venous thromboembolic and bleeding risk in population with obesity hospitalized for an acute medical illness receiving enoxaparin for thromboprophylaxis.

Research and practice in thrombosis and haemostasis·2026
Same journal

Identifying risk factors for venous thromboembolism in medical inpatients: a systematic review and meta-analysis.

Research and practice in thrombosis and haemostasis·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2026

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well
08:01

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

Published on: February 27, 2026

A novel biosensor for measuring plasmin activity.

Ying Dai1,2, Paul Y Kim1, Stefan Heitmeier3

  • 1Thrombosis and Atherosclerosis Research Institute, Hamilton and Departments of Medicine and Medical Sciences, McMaster University, Hamilton, Canada.

Research and Practice in Thrombosis and Haemostasis
|June 15, 2026
PubMed
Summary
This summary is machine-generated.

A novel fluorescence resonance energy quenching-based plasmin sensor (FPS) protein was developed to accurately measure plasmin generation. This advancement aids in assessing fibrinolysis for improved understanding of fibrinolytic disorders in clinical and preclinical research.

Keywords:
antifibrinolytic agentsbiosensing techniquesfibrin clot lysis timefibrinolysinprotein interaction domains and motifs

More Related Videos

The Use of a &#946;-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
10:50

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

Related Experiment Videos

Last Updated: Jun 16, 2026

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well
08:01

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

Published on: February 27, 2026

The Use of a &#946;-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
10:50

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Plasmin is crucial for fibrinolysis, degrading fibrin clots.
  • Accurate measurement of plasmin generation is essential for preclinical and clinical research.
  • Current methods for assessing fibrinolysis require improvement.

Purpose of the Study:

  • To design, synthesize, and evaluate a novel fluorescence resonance energy quenching-based plasmin sensor (FPS) protein.
  • To establish a reliable method for monitoring plasmin generation.
  • To assess the FPS protein's efficiency and specificity in detecting plasmin.

Main Methods:

  • Developed and synthesized a novel FPS protein utilizing fluorescence resonance energy quenching.
  • Monitored FPS hydrolysis via loss of fluorescence resonance energy quenching to assess efficiency and specificity.
  • Employed plasma-based plasmin generation assays to evaluate fibrinolytic cofactors and inhibitors.

Main Results:

  • The FPS protein exhibited higher efficiency for plasmin compared to peptide sensors and was unaffected by plasmin inhibited by α2-macroglobulin.
  • FPS successfully measured plasmin generation in clotting plasma, demonstrating sensitivity to fibrinolytic modulators like α2-antiplasmin, aprotinin, tranexamic acid, and fibrin.
  • Plasmin generation measurements using FPS in healthy volunteers showed consistent results with limited variation.

Conclusions:

  • The developed FPS protein offers a promising tool for evaluating fibrinolysis in both preclinical and clinical settings.
  • FPS can be utilized to measure plasmin generation, aiding in the study of fibrinolytic disorders.
  • This sensor provides a reliable and consistent method for assessing plasmin activity, potentially improving diagnostic and therapeutic strategies.