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Updated: Jun 16, 2026

Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples
Published on: June 6, 2025
[Application Of Next Generation Sequencing To Diagnosis Of Mycobacterium Tuberculosis Drug Resistant Directly From
Khiếu Thị Thúy Ngọc1, Nguyễn Văn Hưng1,2, Trần Huyền Trang1
1Bệnh viện Phổi Trung ương (NLH), Hà Nội.
None:
Rapid identification of antibiotic resistance is essential for effective treatment of Tuberculosis (TB). This study aims to predict drug resistance through whole genome sequencing (WGS) to determine drug resistance in Mycobacterium tuberculosis from 100 sputum samples of TB patients with Xpert MTB positive and Rifampicin resistant to examined at the Central Lung Hospital and Hanoi Lung Hospital from 2020 - 2022. After eliminating samples that did not meet quality assurance, the remaining 38 samples which had quality control (QC) results greater than or equal to 80% and this sample had complete phenotypic and genotypic drug resistance results to analyze and evaluate diagnostic effectiveness. WGS directly from sputum samples to detect drug resistance has sensitivity and specificity for each drug respectively of Isoniazid 91.7% and 100%; Rifampicin 86.79% and 25.0%; Ethambutol 89.5% and 68.4%; Pyrazinamide 56.5% and 100%; Streptomycin 67.7% and 85.7%. Moxifloxacin 50.0% and 96.4%. Whole-genome sequencing testing from sputum samples shows the effectiveness in applying modern molecular biology technology to early diagnosis of drug-resistant tuberculosis, moving towards individualizing treatment regimens suitable for each individual. patients, increasing the rate of successful treatment of drug-resistant tuberculosis in Vietnam.
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