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A TaMYB2-TaMAP3K17 module enhances drought tolerance by promoting reactive oxygen species scavenging in wheat
Shuhui Yang1, Zehao Hou1,2, Lei Zheng2
1MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province)/Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, College of Agriculture, Yangtze University, Jingzhou, 434025, China.
Abstract:
Plant growth and production are strongly affected by water deficit. Mitogen-activated protein kinase kinase kinases (MAP3Ks) are essential signaling components for responses to abiotic stress in many plants. However, the molecular mechanisms underlying their roles in drought-stress tolerance in wheat (Triticum aestivum) remain unclear. In this study, we identified TaMAP3K17 as a drought-induced MAP3K gene in wheat and demonstrated that it encodes a protein localized to both the plasma membrane and nucleus. TaMAP3K17-silenced plants generated by virus-induced gene silencing (VIGS) were hypersensitive to drought stress and accumulated higher levels of reactive oxygen species (ROS) compared to the wild type. TaMAP3K17 overexpression enhanced drought-stress tolerance in transgenic wheat plants. Yeast one-hybrid and dual-luciferase reporter assays showed that TaMYB2 activates the transcription of TaMAP3K17. Suppressing TaMYB2 expression by VIGS resulted in ROS generation and malondialdehyde accumulation in wheat under water-deficit conditions. These findings suggest that a TaMYB2-TaMAP3K17 regulatory module is involved in ROS homeostasis and drought responses in wheat.
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