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Updated: Jun 16, 2026

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
11:56

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

Published on: September 28, 2017

Small-Molecule Kinase Inhibitors Modulating Circadian Rhythms

Irene Castellino1,2, Manuela Bartolini2, Rudolf Andrys1

  • 1Department of Chemistry, Faculty of Science, University of Hradec Kralove, Hradec Kralove, Czech Republic.

Medicinal Research Reviews
|June 15, 2026
PubMed

Insights

Kinase inhibitors show promise for treating circadian clock disorders by targeting phosphorylation in the cellular feedback loop. Further research is needed to bridge the gap between lab results and effective in vivo treatments.

Area of Science:

  • Biochemistry
  • Chronobiology
  • Pharmacology

Background:

  • The circadian clock regulates vital physiological processes via a transcriptional-translational feedback loop.
  • Phosphorylation is critical for maintaining circadian rhythm accuracy within cells.
  • Dysregulation of the circadian clock is linked to various diseases.

Purpose of the Study:

  • To review current kinase inhibitors affecting the mammalian circadian clock.
  • To identify promising therapeutic targets and limitations of existing inhibitors.
  • To guide future research for novel treatments of circadian-related disorders.

Main Methods:

  • Literature review of kinase inhibitors and their effects on the circadian clock.
  • Analysis of phosphorylation's role in circadian rhythm regulation.
  • Evaluation of in vitro activity versus in vivo effectiveness.

Main Results:

  • Kinase inhibitors are potential modulators of the circadian clock.
  • Significant discrepancies exist between in vitro and in vivo efficacy of inhibitors.
  • Specific targets and limitations of current compounds are highlighted.

Conclusions:

  • Kinase inhibitors offer a promising avenue for treating circadian rhythm disorders.
  • Rigorous in vivo validation is essential to translate therapeutic potential into clinical applications.
  • Further development is needed to overcome limitations and ensure effective treatments.
Keywords:
circadian clockcircadian rhythminhibitorkinase

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