Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Blood-brain Barrier00:49

The Blood-brain Barrier

Overview
Cerebral Edema ll: Pathophysiology01:22

Cerebral Edema ll: Pathophysiology

Vasogenic edema is a major form of cerebral edema characterized by abnormal accumulation of fluid in the brain’s extracellular space due to disruption of the blood–brain barrier (BBB). The BBB is a specialized structure composed of endothelial cells connected by tight junctions, supported by astrocytic endfeet and a basement membrane. Under normal conditions, it tightly regulates the movement of ions, proteins, and solutes between the bloodstream and brain parenchyma. When this barrier loses...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sesamin protects SH-SY5Y cells against mechanical stretch injury and promoting cell survival.

BMC neuroscience·2017
Same author

[Hemodynamic effects of synchronous and asynchronous independent lung ventilation with different levels of positive end-expiratory pressure and tidal volumes on unilateral lung injury in dogs].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2010
Same author

[Study on the immuno-effects and influencing factors of Chinese hamster ovary (CHO) cell hepatitis B vaccine among adults, under different dosages].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2010
Same author

Assemblies of fluorine containing bent-shaped liquid crystal molecules studied by using scanning tunneling microscopy.

Journal of nanoscience and nanotechnology·2010
Same author

Carbon nanotubes induce secondary structure changes of bovine albumin in aqueous phase.

Journal of nanoscience and nanotechnology·2010
Same author

[Immunogenicity and protective efficacy of pertactin recombinants against Bordetella bronchiseptica challenge].

Wei sheng wu xue bao = Acta microbiologica Sinica·2010
Same journal

Nanozyme-Reinforced miR-197-3p Delivery Resets Metabolic and Senescence Pathways to Rejuvenate Osteoarthritic Cartilage.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

Correction to "Nanoparticles (NPs)-Meditated LncRNA AFAP1-AS1 Silencing to Block Wnt/β-Catenin Signaling Pathway for Synergistic Reversal of Radioresistance and Effective Cancer Radiotherapy".

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

Femtosecond-Laser Nanocavitation Regenerates SERS-Active Plasmonic Nanogaps for Longitudinal Molecular Sensing at Biointerfaces.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

Correction to "Bioinspired Polyacrylic Acid-Based Dressing: Wet Adhesive, Self-Healing, and Multi-Biofunctional Coacervate Hydrogel Accelerates Wound Healing".

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

Non-Line-of-Sight Passive Ammonia Sensor Loaded With MXene/In<sub>2</sub>O<sub>3</sub> Composites for Agricultural Products Quality Deterioration Detection.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

Cerium Nanoparticle-Mediated Inhibition of the NSUN2/m<sup>5</sup>C Axis Suppresses Synovial Aggression in Rheumatoid Arthritis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2026

Stretch in Brain Microvascular Endothelial Cells (cEND) as an In Vitro Traumatic Brain Injury Model of the Blood Brain Barrier
07:19

Stretch in Brain Microvascular Endothelial Cells (cEND) as an In Vitro Traumatic Brain Injury Model of the Blood Brain Barrier

Published on: October 26, 2013

GPR124 Alleviates Blood-Brain Barrier Disruption by Enhancing Microvascular Endothelial Function after Traumatic

Chen Wang1, Lin Cai1, Qiuyuan Gong1

  • 1Department of Neurosurgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|June 15, 2026
PubMed
Summary
This summary is machine-generated.

G protein-coupled receptor 124 (GPR124) is crucial for neurological recovery after traumatic brain injury (TBI). It maintains blood-brain barrier integrity via the Wnt/β-catenin pathway, offering a potential therapeutic target for TBI.

Keywords:
GPR124Wnt/β‐catenin signalingblood–brain barrierendothelial cellstraumatic brain injury

More Related Videos

Improved Method for the Preparation of a Human Cell-based, Contact Model of the Blood-Brain Barrier
08:40

Improved Method for the Preparation of a Human Cell-based, Contact Model of the Blood-Brain Barrier

Published on: November 12, 2013

Reconstruction of the Blood-Brain Barrier In Vitro to Model and Therapeutically Target Neurological Disease
06:19

Reconstruction of the Blood-Brain Barrier In Vitro to Model and Therapeutically Target Neurological Disease

Published on: October 20, 2023

Related Experiment Videos

Last Updated: Jun 16, 2026

Stretch in Brain Microvascular Endothelial Cells (cEND) as an In Vitro Traumatic Brain Injury Model of the Blood Brain Barrier
07:19

Stretch in Brain Microvascular Endothelial Cells (cEND) as an In Vitro Traumatic Brain Injury Model of the Blood Brain Barrier

Published on: October 26, 2013

Improved Method for the Preparation of a Human Cell-based, Contact Model of the Blood-Brain Barrier
08:40

Improved Method for the Preparation of a Human Cell-based, Contact Model of the Blood-Brain Barrier

Published on: November 12, 2013

Reconstruction of the Blood-Brain Barrier In Vitro to Model and Therapeutically Target Neurological Disease
06:19

Reconstruction of the Blood-Brain Barrier In Vitro to Model and Therapeutically Target Neurological Disease

Published on: October 20, 2023

Area of Science:

  • Neuroscience
  • Vascular Biology
  • Molecular Medicine

Background:

  • Traumatic brain injury (TBI) is a significant global health concern, particularly among young adults.
  • The role of G protein-coupled receptor 124 (GPR124) in the nervous system is established, but its specific involvement in TBI pathophysiology was unknown.
  • GPR124 is recognized as a key regulator within the nervous system.

Purpose of the Study:

  • To investigate the expression and function of GPR124 in the context of TBI.
  • To elucidate the molecular mechanisms by which GPR124 influences neurological recovery and blood-brain barrier (BBB) integrity post-TBI.
  • To explore GPR124 as a potential therapeutic target for TBI.

Main Methods:

  • Utilized a controlled cortical impact (CCI) model of TBI combined with single-cell RNA sequencing to assess GPR124 expression.
  • Employed endothelial cell (EC)-specific conditional knockout (CKO) GPR124 mice for behavioral analyses post-TBI.
  • Established a stretch injury (SI) model to evaluate GPR124's effect on ECs in vitro and in vivo; utilized mass spectrometry and immunoprecipitation.

Main Results:

  • Neurological recovery was significantly impaired in mice lacking GPR124 specifically in endothelial cells after TBI.
  • GPR124 knockdown compromised EC function in SI models and disrupted tight junction integrity in vivo and in vitro.
  • GPR124 was found to interact with fibroblast growth factor binding protein-1, activating the Wnt/β-catenin pathway.

Conclusions:

  • GPR124 is essential for maintaining microvascular endothelial function and blood-brain barrier integrity following TBI.
  • The GPR124-mediated activation of the Wnt/β-catenin pathway is a critical mechanism for improving TBI outcomes.
  • GPR124 represents a promising therapeutic target for enhancing recovery from traumatic brain injury.