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Draft genome of Orthopoxvirus monkeypox virus hMpxV/Uganda/MUWRP-H1922SL/2025 detected in Uganda
Stephen Alafi1, Godfrey Wokorach1,2, Titus Tugume1
1Makerere University Walter Reed Program, Kampala, Uganda.
Abstract:
Mpox virus from Clade I exhibits high virulence and poses significant mortality risks, especially among children. We isolated a strain of the mpox virus (hMpxV/Uganda/MUWRP-H1922SL/2025) from an adult woman in Uganda. Using phylogenetic tree analysis and PoxServer evaluation, we determined that this strain belongs to Clade/Lineage Ib.
Insights
Highly virulent Clade I mpox virus poses risks. A new mpox virus strain (hMpxV/Uganda/MUWRP-H1922SL/2025) isolated in Uganda was identified as Clade/Lineage Ib.
Area of Science:
- Virology
- Epidemiology
- Genomics
Background:
- Clade I mpox virus strains are highly virulent, presenting significant mortality risks, particularly in pediatric populations.
- Mpox virus outbreaks necessitate continuous genomic surveillance to understand viral evolution and transmission dynamics.
Purpose of the Study:
- To characterize a newly isolated mpox virus strain from Uganda.
- To determine the phylogenetic classification of the isolated mpox virus strain.
Main Methods:
- Isolation of the mpox virus strain hMpxV/Uganda/MUWRP-H1922SL/2025 from an adult female in Uganda.
- Phylogenetic tree analysis to ascertain the evolutionary relationships of the virus.
- PoxServer evaluation for precise lineage determination.
Main Results:
- The isolated mpox virus strain, hMpxV/Uganda/MUWRP-H1922SL/2025, was successfully obtained from a Ugandan patient.
- Phylogenetic analysis and PoxServer evaluation confirmed the strain belongs to Clade/Lineage Ib.
Conclusions:
- The identification of a Clade/Lineage Ib mpox virus strain in Uganda contributes to the understanding of mpox virus geographical distribution and genetic diversity.
- This finding underscores the importance of ongoing surveillance for mpox virus, especially in regions where Clade I strains may circulate.
