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  1. Home
  3. Hidden Fungal Dna Structures May Shape Sequencing Outcomes.
  1. Home
  3. Hidden Fungal Dna Structures May Shape Sequencing Outcomes.

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Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
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Hidden Fungal DNA Structures May Shape Sequencing Outcomes.

Paul W Thomas1,2

  • 1Faculty of Natural Sciences, University of Stirling, Stirling, UK.

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
|June 15, 2026

View abstract on PubMed

Summary
This summary is machine-generated.

Fungal DNA is often missed in sequencing due to cell walls and complex structures. Understanding these challenges can improve DNA detection and fungal biology research.

Keywords:
DNAfunginanoporenovel structuressequence artefacts

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Area of Science:

  • Mycology
  • Genomics
  • Bioinformatics

Background:

  • Shotgun metagenomics frequently under-detects fungal DNA.
  • Physical barriers like melanized cell walls and complex DNA structures may impede detection.
  • Oxford Nanopore Technologies sequencing faces challenges with native fungal DNA, including pore clogging and translocation issues.

Purpose of the Study:

  • To investigate the reasons behind the under-detection of fungal DNA in metagenomic sequencing.
  • To explore the impact of fungal DNA structural complexities on sequencing technologies like Oxford Nanopore.
  • To enhance the accuracy and scope of fungal DNA detection in various biological samples.

Main Methods:

  • Analysis of under-detection patterns in shotgun metagenomic datasets.
  • Investigation of Oxford Nanopore Technologies sequencing behavior with native fungal DNA.
  • Characterization of physical and structural properties of fungal DNA relevant to sequencing.

    • Main Results:

    • Fungal DNA under-detection is linked to cell wall composition and DNA conformation.
    • Specific structural features of fungal DNA cause pore clogging and aberrant translocation dynamics during Nanopore sequencing.
    • These challenges are likely due to intrinsic, currently undescribed, fungal DNA properties.

    Conclusions:

    • Addressing physical and structural barriers is crucial for improving fungal DNA detection.
    • Further research into fungal DNA architecture can enhance sequencing accuracy.
    • Understanding these sequencing complexities will advance fungal biology and genomics.