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Dye-Decolorizing Peroxidase for Lignin Depolymerization: a Comprehensive Review on Enzyme Structural Characteristics,
Yuheng Xie1, Shufan Zhao1, Feng Guo1
1State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211800, People's Republic of China.
Applied Biochemistry and Biotechnology
|June 15, 2026
Summary
Dye-decolorizing peroxidase (DyP) offers a sustainable method for breaking down lignin, a renewable material. This review explores DyP
Area of Science:
- Biotechnology
- Biochemistry
- Renewable Energy
Background:
- Lignin, a renewable aromatic polymer, presents a sustainable alternative to fossil fuels.
- Biological lignin depolymerization offers mild conditions, low energy consumption, and reduced pollution compared to traditional methods.
- Dye-decolorizing peroxidase (DyP) shows significant potential for bioenzymatic lignin depolymerization.
Purpose of the Study:
- To review the application of dye-decolorizing peroxidase (DyP) in lignin depolymerization.
- To analyze factors influencing DyP-mediated lignin depolymerization.
- To propose strategies for enhancing depolymerization efficiency.
Main Methods:
- Summarized properties of lignin and various DyPs.
- Reviewed lignin depolymerization reactions and influencing factors.
- Evaluated current strategies for improving depolymerization efficiency.
Main Results:
- DyP enzymes exhibit excellent capabilities in breaking down lignin.
- Factors influencing depolymerization efficiency were systematically analyzed.
- Protein engineering and other technologies offer pathways to enhance efficiency.
Conclusions:
- DyP-based bioenzymatic lignin depolymerization is a promising sustainable technology.
- Further research focusing on protein engineering can optimize DyP efficiency for industrial applications.
- This review provides a foundation for advancing DyP applications in lignin valorization.

