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Updated: Jun 16, 2026

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

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Deciphering RNA modification dynamics during spermatogenesis and sperm maturation.

Zheng Cao¹, Zongyu Yu¹, Xin Wang¹

¹Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, 200092, China.

Science China. Life Sciences

|June 15, 2026

Summary

This summary is machine-generated.

RNA modifications are crucial for male fertility. This study maps these modifications during sperm development and maturation, revealing dynamic changes and alterations in a diabetes model.

Keywords:

RNA modification T2DM (type 2 diabetes mellitus)epididymis epigenetic spermatogenesis

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Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

Area of Science:

Reproductive Biology
Epigenetics
Molecular Biology

Background:

RNA modifications play vital roles in male fertility and offspring health.
The dynamic changes in RNA modifications during spermatogenesis and sperm maturation are not fully understood.

Purpose of the Study:

To profile and analyze the dynamic landscape of 27 RNA modifications during mouse spermatogenesis and epididymal maturation.
To investigate the impact of type 2 diabetes on sperm RNA modification signatures.

Main Methods:

Utilized a liquid chromatography-tandem mass spectrometry platform to quantify 27 RNA modifications.
Analyzed RNA modifications in developing testes, isolated spermatogenic cells, and epididymal sperm.
Integrated analyses of RNA modification signatures in a type 2 diabetes mellitus mouse model.

Main Results:

Most RNA modifications are reprogrammed from the testis to the epididymis, declining in mature sperm.
Specific modifications like m6A and m5U are enriched in meiotic cells, while m5C and m2G are abundant in caput sperm.
Diabetes dynamically alters sperm RNA modification signatures and inter-modification networks during epididymal maturation.

Conclusions:

This study delineates the dynamic RNA modification landscape during spermatogenesis and maturation.
Uncovered the origins and alterations of RNA modifications in a type 2 diabetes model.
Provides insights into RNA modification-dependent spermatogenesis and sperm epigenetic remodeling.