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Related Concept Videos

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
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Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 17, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Single-shot lensless dual-mode ultraviolet imaging based on diffuser speckle modulation.

Jiaqi Song, Zi-Qing Zhao, Ming-Fei Li

    Optics Letters
    |June 15, 2026
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a novel dual-mode ultraviolet (UV) lensless imaging technique. It enables simultaneous reflection/transmission and fluorescence imaging in a single shot, simplifying complex UV imaging setups.

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    Published on: December 1, 2023

    Area of Science:

    • Optics and Photonics
    • Materials Science
    • Biomedical Imaging

    Background:

    • Ultraviolet (UV) imaging offers unique insights into material properties and structural details not visible in standard light.
    • Traditional UV imaging methods struggle to simultaneously capture reflection/transmission and fluorescence data without cumbersome optical filters.
    • Existing techniques often require complex optical setups, limiting system compactness and ease of use.

    Purpose of the Study:

    • To develop a simplified, lensless imaging system for simultaneous dual-mode UV imaging.
    • To overcome the limitations of conventional UV imaging in acquiring both reflection/transmission and fluorescence data.
    • To enable the creation of ultra-compact and versatile UV imaging devices.

    Main Methods:

    • A novel dual-mode UV lensless imaging approach utilizing diffuser speckle modulation.
    • Single-shot measurement to acquire both reflection/transmission and UV-excited fluorescence information.
    • Direct reconstruction of both imaging modalities without the need for optical lenses or filters.

    Main Results:

    • Successful demonstration of simultaneous reflection/transmission and fluorescence imaging using a lensless setup.
    • Elimination of the requirement for optical filters, simplifying the imaging system.
    • Achieved an ultra-compact system design compared to conventional lens-based UV imaging.

    Conclusions:

    • The developed diffuser speckle modulation method provides a streamlined approach for dual-mode UV imaging.
    • This lensless technique significantly enhances the practicality and compactness of UV imaging systems.
    • The method holds potential for integration into intelligent UV cameras for advanced computer vision applications.