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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
Published on: November 16, 2019
Single-shot lensless dual-mode ultraviolet imaging based on diffuser speckle modulation
Abstract:
Ultraviolet (UV) imaging can reveal structural flaws, fluorescent markers, and characteristic signals generated by material changes that are difficult to detect in visible-light imaging and has been applied in numerous fields. However, in traditional UV imaging, it is challenging to simultaneously acquire reflection/transmission and UV-excited fluorescence imaging, often requiring additional optical filters to eliminate interference bands. Here, we demonstrate a dual-mode UV lensless imaging method based on diffuser speckle modulation, which enables direct reconstruction of both reflection/transmission and fluorescence images from a single-shot measurement. Compared with conventional methods, our scheme eliminates the need for optical lenses and filters, enabling an ultra-compact lensless system. This approach can be further integrated with other image post-processing operations or general computer vision tasks for the development of task-oriented intelligent UV cameras.
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