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Updated: Jun 17, 2026
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Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
Published on: June 30, 2018
Background-enhanced NIR-II single-pixel imaging for fluorescent localization and dynamic tracking
Optics Letters
|June 15, 2026
Summary
We developed a novel near-infrared-II (NIR-II) imaging system for precise fluorescent localization and dynamic tracking. This background-enhanced single-pixel system overcomes limitations of conventional methods, improving surgical navigation.
Area of Science:
- Biomedical Imaging
- Optical Engineering
- Medical Technology
Background:
- Near-infrared-II (NIR-II) fluorescence imaging provides high contrast but lacks morphological context for accurate pathological localization.
- Conventional image fusion methods using heterogeneous cameras introduce parallax and registration errors, hindering precise localization.
- Accurate localization and dynamic tracking of fluorescent signals are crucial for advanced surgical navigation.
Purpose of the Study:
- To develop a novel background-enhanced single-pixel imaging system for high-fidelity NIR-II fluorescence localization and dynamic tracking.
- To overcome the limitations of conventional fusion techniques and improve the precision of pathological localization.
- To provide a robust solution for real-time surgical navigation by integrating fluorescence imaging with anatomical context.
Main Methods:
- Implemented a background-enhanced NIR-II single-pixel imaging system utilizing a shared spatial encoding strategy.
- Employed parallel silicon and InGaAs detectors to eliminate geometric distortions between background and fluorescence images.
- Integrated a bowl-shaped detector to enhance light collection efficiency for improved signal-to-noise ratio in dynamic scenes.
Main Results:
- Achieved precise fluorescent localization and dynamic tracking without additional alignment algorithms by eliminating geometric distortions.
- Demonstrated a ~7-fold signal-to-noise ratio improvement through enhanced light collection efficiency.
- Enabled dynamic fluorescence tracking at 10 frames per second with a 128 × 128 physical pixel resolution.
Conclusions:
- The proposed novel NIR-II imaging architecture enables precise localization and dynamic tracking of fluorescent signals within an anatomical background.
- The system effectively overcomes parallax and registration errors inherent in conventional fusion methods.
- This technology offers a promising new solution for enhanced surgical navigation and pathological localization.
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