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Updated: Jun 17, 2026

Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model
Published on: February 14, 2021
Lonicera japonica polysaccharide restores mucosal integrity in ulcerative colitis by increasing microbiota-derived
Xinyu Bai1, Xiuming Rao2, Keke Tian2
1Department of Anesthesiology, Second Affiliated Hospital of Army Medical University, Chongqing, China; Yu-Yue Pathology Scientific Research Center, Jinfeng Laboratory, Chongqing, China.
Abstract:
WLJP-025p, a homogeneous polysaccharide from Lonicera japonica Thunb., alleviates experimental ulcerative colitis in mice by reshaping the gut microbiota and restoring intestinal spermidine. Microbial fermentation, microbiota depletion, and microbiota transplantation support a microbiota-dependent mechanism. Microbiota-derived spermidine interacts with HADHA and partially reverses inflammatory HADHA-associated metabolic reprogramming, as shown by restored fatty acid oxidation (FAO), reduced lactate accumulation, improved basal respiration and etomoxir-sensitive oxygen consumption rate (OCR), and preserved epithelial integrity in Caco-2 cells and intestinal organoids. In vivo, WLJP-025p dose-dependently reduces colitis pathology and inflammation, partially reverses FAO-related metabolic disruption, and promotes epithelial maturation and barrier integrity. Serum biochemical and protein analyses further support reduced systemic inflammation and improved mucosal homeostasis. These findings define a microbiota-derived spermidine-HADHA axis that supports mucosal homeostasis in ulcerative colitis.
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