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Related Experiment Video

Updated: Jun 17, 2026

In vivo Calcium Imaging in Mouse Inferior Olive
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In vivo Calcium Imaging in Mouse Inferior Olive

Published on: June 10, 2021

Region-Specific Lens Insertion Strategies For In vivo Calcium Imaging In Mice Across Cortical And Hippocampal

Jonghan Shin1, Jee Hyun Yi2, Min Whan Jung3

  • 1Department of Biological Sciences, Korea Advanced Institute of Science and Technology.

Journal of Visualized Experiments : Jove
|June 15, 2026
PubMed
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This protocol details region-specific surgical techniques for implanting optical lenses for in vivo calcium imaging in the cortex and hippocampus. It aims to improve surgical success and imaging quality by addressing anatomical variations and reducing trial-and-error.

Area of Science:

  • Neuroscience
  • Surgical Techniques
  • Optical Imaging

Background:

  • In vivo calcium imaging relies on surgically implanted optical lenses to monitor neuronal activity.
  • Surgical success and imaging quality are constrained by brain region-specific anatomy.
  • Current methods often lack detailed, region-specific surgical guidance.

Purpose of the Study:

  • To provide a detailed, region-specific protocol for optical lens implantation in cortical and hippocampal areas.
  • To standardize surgical procedures for improved reliability and reproducibility in neuroscience research.
  • To guide the selection and implantation of lenses based on anatomical considerations.

Main Methods:

  • Describes a surgical workflow including target localization, skull leveling, and region-specific craniotomy.

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Related Experiment Videos

Last Updated: Jun 17, 2026

In vivo Calcium Imaging in Mouse Inferior Olive
08:58

In vivo Calcium Imaging in Mouse Inferior Olive

Published on: June 10, 2021

In Vivo Calcium Imaging of Granule Cells in the Dentate Gyrus of Hippocampus in Mice
07:00

In Vivo Calcium Imaging of Granule Cells in the Dentate Gyrus of Hippocampus in Mice

Published on: August 2, 2024

  • Details three distinct implantation strategies: conventional (ventral hippocampus), tissue aspiration (dorsal hippocampus), and prism lens (cortical).
  • Provides guidance on selecting surgical parameters like lens diameter, craniotomy size, and fixation methods based on tissue depth and vascular architecture.

    • Main Results:

    • The protocol explicitly documents underreported surgical considerations for lens implantation.
    • It offers practical guidance to minimize trial-and-error in lens implantation procedures.
    • Demonstrates region-specific strategies for successful optical lens implantation across different brain circuits.

    Conclusions:

    • This protocol enhances the reliability and reproducibility of in vivo calcium imaging experiments.
    • It provides a practical framework for selecting and implementing appropriate lens implantation strategies.
    • Facilitates more consistent neuronal population activity monitoring in cortical and hippocampal research.