Efficient Isolation of Adipose-derived Stem Cells and Adipocytes from Porcine Adipose Tissue

Adipose-derived stem cells (ADSCs) have emerged as ideal seed cells in regenerative medicine due to their abundant sources, minimally invasive harvesting, multi-lineage differentiation potential, and immunomodulatory properties. Their applications span tissue repair, disease modelling, and cell therapy; however, efficient isolation of high-viability ADSCs remains critical for advancing research and clinical translation. Conventional isolation methods, such as enzymatic digestion combined with mechanical dissociation via pipetting, are limited by lengthy processing times (1-3 h), poor cell viability (often <70%), and substantial batch-to-batch variability, compromising downstream experiments. Here, we present the SoniConvert system (a mechanical wave-based cell separation system), which combines mechanical wave and enzymatic digestion to address these challenges. The system integrates a microprocessor-based control unit that regulates mechanical wave-mediated dissociation via digital feedback, together with a tissue-specific loosening reagent optimized for adipose tissue (5-15 min incubation). This approach enables rapid conversion of tissue to a single-cell suspension, with mechanical dissociation completed in 3-9 s. The system offers three advantages: (1) ultra-fast processing, with the core isolation process-from enzymatic digestion to initial cell fraction separation-completed in approximately 30 min (>90% reduction compared to conventional methods); (2) high viability preservation, with trypan blue staining confirming cell viability of 80-95%, exceeding traditional protocols; and (3) broad compatibility, as the resulting single-cell suspensions meet requirements for primary cell culture, flow cytometry, cytotoxicity assays, and adipose organoid construction. By reducing processing time, enhancing cell integrity, and limiting variability, this system provides a practical platform for adipose-related research.