Aseptic Practices in Non-Surgical Endodontics: A Survey Across 3 Practitioner Groups

This study aimed to evaluate aseptic practices during non-surgical root canal treatment across general dental practitioners (GDPs), postgraduate endodontic residents (ERs) and specialist endodontists (SEs).

A descriptive, cross-sectional survey was conducted online between September 2024 and January 2025. Sixteen closed-ended questions assessed adherence to asepsis, focusing on dental dam, gloves, endodontic files, examination instruments and obturation supplies. Responses were analysed by professional group using chi-square tests, with post-hoc z-tests when relevant. Given the exploratory nature of the study, p-values < 0.05 were interpreted descriptively.

A total of 815 participants completed the survey (SEs: 49.1%, GDPs: 36.4%, ERs: 14.5%). Systematic dental dam use was highest among SEs (97.2%) and lowest among GDPs (78.1%, p < 0.001). Disinfection of the dental dam after placement and before obturation was inconsistent, though SEs reported greater adherence. Glove changes and replacement of examination instruments were inconsistently performed, especially prior to obturation. Precurving files with gloves was reported by 54.4% of respondents. Gutta-percha disinfection prior to obturation was less frequent among GDPs than SEs and ERs (p < 0.001). Overall, SEs demonstrated better adherence to aseptic practices, though no group achieved complete compliance.

Aseptic practices during non-surgical root canal treatment remain variable, with critical steps often neglected, even among specialists. Clearer guidelines and consistent education are needed, emphasising asepsis throughout treatment. Effective measures are simple, cost-effective and readily implementable in daily practice.