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Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

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Integrated CRISPR-Cas12a-Based Biosensors with Subwavelength Grating Microring Resonators for Ultrasensitive

Zi-Meng Zhang^1,2, Chang Xu³, Yao Zhu⁴

¹School of Integrated Circuits, Harbin Institute of Technology, Shenzhen 518055, China.

|June 15, 2026

Summary

This study presents a novel photonic biosensor using CRISPR-Cas12a and microring resonators for ultrasensitive nucleic acid detection. It achieves high specificity and sensitivity, enabling rapid identification of viral mutations like SARS-CoV-2 variants.

Keywords:

CRISPR biosensors mutation detection optical resonance subwavelength grating

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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

Area of Science:

Biotechnology
Nanotechnology
Molecular Diagnostics

Background:

Nucleic acid detection is vital for identifying viral mutations but faces challenges in speed, precision, and sensitivity with current methods.
Conventional diagnostics like PCR and NGS are complex, while existing photonic biosensors often lack specificity and sensitivity.

Purpose of the Study:

To develop an integrated photonic biosensing platform for ultrasensitive and mutation-resolved molecular diagnostics.
To synergize CRISPR-Cas12a's programmable recognition with the sensitivity of subwavelength grating microring resonators.

Main Methods:

Functionalized sensor surface with DNA probes and gold nanoparticles.
Utilized CRISPR-Cas12a for target recognition and probe cleavage, releasing nanoparticles.
Employed subwavelength grating microring resonators for quantifiable resonance wavelength shift, amplified by photothermal effect.

Main Results:

Achieved a detection limit of 0.7 fM for SARS-CoV-2 variants, a four-order-of-magnitude improvement over fluorescence-based CRISPR assays.
Successfully discriminated between wild-type, Delta, and Omicron strains of SARS-CoV-2.
Demonstrated a generalizable platform for ultrasensitive molecular diagnostics on a CMOS-compatible photonic chip.

Conclusions:

The developed platform offers a significant advancement in ultrasensitive, mutation-resolved molecular diagnostics.
This technology paves the way for improved point-of-care testing and genomic surveillance.
The integrated photonic biosensor demonstrates high specificity and sensitivity for detecting viral mutations.