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Related Experiment Video

Updated: Jun 17, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Intelligent logic-gate-integrated capillary fluorescence imprinted microarray sensor for rapid simultaneous detection

Ao Ma¹, Zhihui Liao¹, Ruoyan Wang¹

¹School of Pharmaceutical Sciences, Jishou University, Hunan, 416000, PR China; Key Laboratory of Medicinal Resources Chemistry and Pharmacology in Wuling Mountainous of Hunan Province College, Jishou University, Jishou, 416000, PR China.

|June 15, 2026

Summary

A novel capillary fluorescence microarray sensor rapidly detects endocrine disrupting compounds (EDCs) like DBP, BPA, and TPHP. This intelligent sensor offers a practical solution for simultaneous, on-site EDC analysis in various samples.

Keywords:

Endocrine disrupting compounds Fluorescence capillary Imprinted Microarray sensor One-click image processing

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Area of Science:

Environmental Science
Analytical Chemistry
Biotechnology

Background:

Endocrine disrupting compounds (EDCs) pose significant environmental and human health risks due to their persistence and toxicity.
Existing detection methods for EDCs can be time-consuming and lack the ability for simultaneous multi-analyte detection.

Purpose of the Study:

To develop a rapid, visual, and intelligent sensor for the simultaneous identification and quantification of multiple EDCs.
To create a sensitive and selective microarray sensor for detecting dibutyl phthalate (DBP), bisphenol A (BPA), and triphenyl phosphate (TPHP).

Main Methods:

Construction of a capillary fluorescence imprinted microarray sensor using molecularly imprinted polymers.
Utilized a static gas-driven coating approach for polymer modification within capillaries.
Integrated fluorescence sensing with intelligent logic gates and one-step image analysis for detection.

Main Results:

The sensor achieved rapid detection of DBP, BPA, and TPHP within 3.5 minutes.
Demonstrated favorable sensitivities for DBP (0.65 nM), BPA (7.2 nM), and TPHP (4.6 nM).
Successfully identified target EDCs in spiked biological and food samples, showcasing high practicability.

Conclusions:

The developed capillary fluorescence microarray sensor provides a promising strategy for on-site, simultaneous, and rapid detection of multiple EDCs.
The intelligent logic gate integration enhances anti-interference capabilities and detection intelligence.
The sensor requires only a microvolume of sample (50 μL), making it efficient for real-world applications.