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Updated: Jun 17, 2026
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Visual Detection of Multiple Nucleic Acids in a Capillary Array
Published on: November 15, 2017
Intelligent logic-gate-integrated capillary fluorescence imprinted microarray sensor for rapid simultaneous detection
Ao Ma1, Zhihui Liao1, Ruoyan Wang1
1School of Pharmaceutical Sciences, Jishou University, Hunan, 416000, PR China; Key Laboratory of Medicinal Resources Chemistry and Pharmacology in Wuling Mountainous of Hunan Province College, Jishou University, Jishou, 416000, PR China.
Talanta
|June 15, 2026
Summary
A novel capillary fluorescence microarray sensor rapidly detects endocrine disrupting compounds (EDCs) like DBP, BPA, and TPHP. This intelligent sensor offers a practical solution for simultaneous, on-site EDC analysis in various samples.
Area of Science:
- Environmental Science
- Analytical Chemistry
- Biotechnology
Background:
- Endocrine disrupting compounds (EDCs) pose significant environmental and human health risks due to their persistence and toxicity.
- Existing detection methods for EDCs can be time-consuming and lack the ability for simultaneous multi-analyte detection.
Purpose of the Study:
- To develop a rapid, visual, and intelligent sensor for the simultaneous identification and quantification of multiple EDCs.
- To create a sensitive and selective microarray sensor for detecting dibutyl phthalate (DBP), bisphenol A (BPA), and triphenyl phosphate (TPHP).
Main Methods:
- Construction of a capillary fluorescence imprinted microarray sensor using molecularly imprinted polymers.
- Utilized a static gas-driven coating approach for polymer modification within capillaries.
- Integrated fluorescence sensing with intelligent logic gates and one-step image analysis for detection.
Main Results:
- The sensor achieved rapid detection of DBP, BPA, and TPHP within 3.5 minutes.
- Demonstrated favorable sensitivities for DBP (0.65 nM), BPA (7.2 nM), and TPHP (4.6 nM).
- Successfully identified target EDCs in spiked biological and food samples, showcasing high practicability.
Conclusions:
- The developed capillary fluorescence microarray sensor provides a promising strategy for on-site, simultaneous, and rapid detection of multiple EDCs.
- The intelligent logic gate integration enhances anti-interference capabilities and detection intelligence.
- The sensor requires only a microvolume of sample (50 μL), making it efficient for real-world applications.
