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Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues
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[Examining Wang Jiufeng and his works].

Y P Zhao1, Q W Shi1, C Y Wu1

  • 1Institute of Chinese Medicine Literature, Nanjing University of Chinese Medicine, Nanjing 210023, China.

Zhonghua Yi Shi Za Zhi (Beijing, China : 1980)
|June 15, 2026
PubMed
Summary

This study clarifies the life and works of Qing Dynasty physician Wang Jiufeng, resolving historical inconsistencies. Findings confirm there was only one prominent Wang Jiufeng, a respected healer.

Area of Science:

  • History of Medicine
  • Chinese Medical History
  • Qing Dynasty Studies

Background:

  • Wang Jiufeng was a notable physician in Zhenjiang during the Qing Dynasty's Qianjia period.
  • Existing historical records regarding Wang Jiufeng's life and contributions are scarce and often contradictory.
  • His transition from Confucianism to medicine highlights a significant career shift in historical Chinese scholarship.

Purpose of the Study:

  • To meticulously examine Wang Jiufeng's family background, life events, associates, and medical works.
  • To resolve historical disputes concerning Wang Jiufeng's identity, including the 'North' and 'South' Wang Jiufeng distinction.
  • To clarify contentious details such as his death year, alias Mingjing, deafness etiology, and official appointments.

Main Methods:

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  • Primary source analysis utilizing Wang Jiufeng's self-compiled genealogy.
  • Comparative literature review to address inconsistencies in existing historical materials.
  • Biographical research focusing on historical physicians of the Qing Dynasty.
  • Main Results:

    • Established the existence of a single, prominent physician named Wang Jiufeng during the Qianjia period.
    • Differentiated Wang Jiufeng from other historical figures potentially confused with him.
    • Resolved specific historical debates regarding his personal life and career.

    Conclusions:

    • This research consolidates fragmented historical data, presenting a unified biography of Wang Jiufeng.
    • The study confirms Wang Jiufeng's singular identity and significant standing as a physician in Zhenjiang.
    • Provides a clearer understanding of a key figure in Qing Dynasty medical history.