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Related Experiment Video

Updated: Jun 17, 2026

An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses

An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses

Published on: November 28, 2010

Ovarian localization drives IL-7R-dependent CD8 coreceptor expression in peripheral double-negative T cells.

Toni Martin¹, Howard A Young¹, Enitome E Bafor^2,3

¹Cancer Innovation Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD, 21702, USA.

Cellular and Molecular Life Sciences : CMLS

|June 15, 2026

Summary

The ovary actively conditions T cells, instructing peripheral double-negative T cells (DNTs) to express CD8 upon entry. This immune conditioning requires IL-7 receptor signaling for efficient induction.

Keywords:

Immune homeostasis Lymphocyte adaptation Ovarian immunity Reproductive immunology T cell plasticity Tissue conditioning

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Published on: August 2, 2024

Area of Science:

Immunology
Reproductive Biology
T cell Biology

Background:

The ovary is immunologically active, balancing inflammation and T cell tolerance.
It is unclear if the ovary instructs infiltrating lymphocytes or simply enriches existing subsets.

Purpose of the Study:

To investigate if the ovary actively modifies the phenotype of infiltrating T cells.
To identify the mechanisms behind ovarian immune cell conditioning.

Main Methods:

Adoptive transfer of labeled splenic double-negative T cells (DNTs) into recipient mice.
Analysis of DNT phenotype and localization in the ovary using flow cytometry and single-cell RNA-sequencing.
In vitro co-culture assays with ovarian cells and DNTs.
Assessment of IL-7 receptor (IL-7R) and IL-7 roles in DNT CD8 upregulation.

Main Results:

Ovarian localization led to CD8 expression on transferred DNTs within 4 days.
Ovarian cells in vitro induced CD8 upregulation on DNTs.
Single-cell RNA-seq revealed IL-7-expressing stromal cells and IL-7R-enriched DNTs in the ovary.
IL-7R signaling was essential for efficient CD8 induction on DNTs in the ovary.

Conclusions:

The ovary acts as an immune-conditioning environment.
Ovarian entry induces CD8 coreceptor expression on peripheral DNTs.
IL-7R signaling is critical for this T cell phenotype modulation in the ovarian microenvironment.