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Updated: Jun 17, 2026

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Ensilication preserves high-molecular weight native DNA for clinical long-read sequencing.

Alexis Ferrasse¹, Rodrigo Mendez^1,2, John E Gorzynski^1,2,3

¹Department of Medicine, Division of Cardiovascular Medicine, Stanford University, Stanford, CA, USA.

|June 16, 2026

Summary

Ensilication preserves DNA for native long-read sequencing at ambient temperature for 30 days. This method maintains DNA integrity and sequencing performance without cold storage, enabling wider access to genetic and epigenetic analysis.

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Last Updated: Jun 17, 2026

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Published on: March 31, 2017

Area of Science:

Genomics
Molecular Biology
Biotechnology

Background:

Native long-read DNA sequencing captures genetic variants and epigenetic modifications.
Current methods rely on cold-chain infrastructure, limiting accessibility.

Purpose of the Study:

To evaluate ensilication as a cold-chain-free preservation method for native long-read DNA sequencing.
To assess the impact of ambient temperature preservation on DNA integrity, sequence accuracy, and methylation information.

Main Methods:

DNA samples were preserved using ensilication at ambient temperature for 30 days.
Sequencing performance was compared to conventionally frozen samples (-80°C).
Variant calling accuracy and genome-wide CpG methylation were analyzed across reference genomes.

Main Results:

Ensilication preserved DNA at ambient temperature for 30 days with sequencing performance comparable to -80°C freezing.
No significant differences were observed in read length, variant-calling accuracy, or CpG methylation between ensilicated and frozen samples.
Ensilicated DNA showed improved handling tolerance and fragment integrity under accelerated weathering.

Conclusions:

Ensilication enables diagnostic-quality native long-read sequencing without cold-chain infrastructure.
Ambient storage and transport of DNA are feasible while preserving sequence and methylation information.
This approach expands access to advanced sequencing technologies in resource-limited settings.