Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A zwitterionic-enabled one-pot electrochemical strategy for stable end-point detection of isothermal nucleic acid amplification.

Analytical methods : advancing methods and applications·2026
Same author

Characterization and Engineering of a Novel <i>cis</i>-Prenyltransferase from the Edible Red Alga <i>Porphyra umbilicalis</i> for Polyprenol Biosynthesis.

Journal of agricultural and food chemistry·2026
Same author

3D-Photothermal Ultrafast PCR via rGO/PDMS-Based Microfluidic Chip and Its Application in Rapid Identification of Gram-Negative Bacteria in Whole Blood.

ACS applied materials & interfaces·2026
Same author

Active Polymerase on Metal-Organic Frameworks: Space-Confined Amplification for the Extraction-Free Detection of Pathogens in Blood.

ACS sensors·2026
Same author

Functional and Mechanistic Characterization of VacPT, a Novel <i>cis</i>-Prenyltransferase from <i>Vibrio alginolyticus</i>.

ACS synthetic biology·2026
Same author

MBD-Functionalized Magnetic Nanoparticles for Direct Enrichment of Plasma Methylated DNA in High-Sensitivity Liquid Biopsy.

Analytical chemistry·2026
Same journal

Machine Learning-Assisted Nanopore for Enhanced Fingerprinting Analysis of Functional Glycans.

Analytical chemistry·2026
Same journal

Correction to "Maleylpyruvic Acid-Inducible Gene Expression System and Its Application for the Development of Gentisic Acid Biosensor".

Analytical chemistry·2026
Same journal

Computer-Aided Rational Hapten Design for Broad-Spectrum Monoclonal Antibody Development against Anthraquinones and Its Application in Lateral Flow Immunoassay.

Analytical chemistry·2026
Same journal

One-Step Chemoenzymatic Labeling and Oxime-Reversible Enrichment for O-GlcNAcylation Profiling under Oxidative Stress.

Analytical chemistry·2026
Same journal

Acid/NIR Dual-Responsive Nanoplatform with AND Logic-Gated Controlled Nitric Oxide Release for Companion Theranostics of Tumors.

Analytical chemistry·2026
Same journal

Multicharged Foldable Plasma Membrane Probes for Precise Cancer Cell Discrimination and Fluorescence-Guided Surgery.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 17, 2026

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
08:58

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

An Integrated Rotation-Programmed and Gravity-Driven Microfluidic Platform for Direct Bacterial Detection from Whole

Xiaoguang Lu1, Zishan Qin1, Jiarui Yu1

  • 1Shandong Provincial Key Laboratory of Analytical Chemistry for Life Science and Intelligent Detection, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, Sino-UAE International Cooperative Joint Laboratory of Pathogenic Microorganism Rapid Detection, College of Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.

Analytical Chemistry
|June 16, 2026
PubMed
Summary
This summary is machine-generated.

A novel microfluidic platform automates bacterial DNA extraction and detection for bloodstream infections. This rotation-programmed device simplifies sample processing, achieving results in 1.5 hours with high sensitivity.

More Related Videos

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood
10:13

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Published on: June 3, 2014

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

Related Experiment Videos

Last Updated: Jun 17, 2026

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
08:58

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood
10:13

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Published on: June 3, 2014

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Molecular Diagnostics

Background:

  • Accurate molecular detection of bloodstream infections requires efficient pathogenic DNA purification from complex blood matrices.
  • Existing automated methods often involve complex setups or large fluid volumes, posing practical challenges.

Purpose of the Study:

  • To develop a simplified, automated microfluidic platform for bacterial pathogen detection in whole blood.
  • To integrate DNA extraction, amplification, and detection into a single, gravity-driven system.

Main Methods:

  • A rotation-programmed microfluidic chip utilizing gravity-driven flow was designed.
  • Antibiotic-modified magnetic nanoparticles automated sample processing (adsorption, washing, elution).
  • Loop-mediated isothermal amplification (LAMP) and machine learning-based colorimetric detection were employed.

Main Results:

  • The integrated system achieved automated sample-to-answer processing within 1.5 hours.
  • A limit of detection of 10^2 CFU mL^-1 for *S. aureus* in whole blood was demonstrated.
  • Machine learning enabled rapid and accurate colorimetric result interpretation.

Conclusions:

  • The developed rotation-programmed microfluidic platform offers a simple and efficient solution for automated bloodstream infection diagnostics.
  • This technology has potential for broader applications in complex biological sample processing.