Multiplexed digital colloid-enhanced Raman spectroscopy for metabolite detection via selective molecular affinity

Area of Science:

• Analytical Chemistry
• Biochemistry
• Materials Science

Background:

• Accurate metabolite quantification is crucial for understanding biological processes and disease states.
• Current methods for multiplex metabolite analysis often face limitations in sensitivity and breadth of coverage.
• Developing advanced analytical tools is essential for comprehensive metabolic profiling.

Purpose of the Study:

• To develop a novel dual-nanoparticle platform for broad metabolite capture.
• To integrate this platform with digital colloid-enhanced Raman spectroscopy (d-CEERS).
• To achieve sensitive and multiplexed quantification of metabolites in complex, low-concentration mixtures.

Main Methods:

• Construction of a dual-nanoparticle system with tailored, complementary affinities for diverse metabolites.
• Integration of the nanoparticle platform with digital colloid-enhanced Raman spectroscopy (d-CEERS) for signal enhancement.
• Application of the integrated system to analyze low-concentration metabolite mixtures.

Main Results:

• The dual-nanoparticle platform demonstrated broad affinity for a wide spectrum of metabolites.
• The integrated d-CEERS system achieved high sensitivity in metabolite detection.
• Successful multiplex quantification of multiple metabolites in complex mixtures was demonstrated.

Conclusions:

• The developed dual-nanoparticle platform coupled with d-CEERS offers a sensitive and versatile approach for metabolite analysis.
• This integrated system enables efficient capture and detection of diverse metabolites, even at low concentrations.
• The technology holds promise for advancing metabolic research and diagnostics.