Evolving a fluorescent light-up aptamer for rotationally quenched asymmetric rhodamines
Franziska Grün1, Daniel Englert1, Andrijana Crenner1
1Institute of Pharmacy and Molecular Biotechnology (IPMB), Heidelberg University, Heidelberg, Germany. msunbul@uni-heidelberg.de.
Abstract:
We describe the evolution and characterization of a fluorescent light-up aptamer, RhoBASTG36C, that binds asymmetric, rotationally quenched rhodamines. Binding induces an over 300-fold fluorescence turn-on, which we leveraged for real-time monitoring in vitro transcription reactions and live-cell imaging of tRNA and mRNA in E. coli.


