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To the homeRNAmax: Developing an Improved Blood Self-Collection and Stabilization Platform for Remote Transcriptomic

Madeleine Eakman1, Filip Stefanovic1, Jean Berthier1

  • 1Department of Chemistry, University of Washington, Seattle, Washington 98195, United States.

Analytical Chemistry
|June 16, 2026
PubMed
Summary
This summary is machine-generated.

The homeRNAmax kit enables remote blood collection and stabilizes RNA for gene expression analysis. This user-friendly device improves upon previous designs, facilitating easier participation in research studies.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genomics

Background:

  • Decentralizing human subjects research to participants' homes reduces participation barriers.
  • Previous homeRNA kit stabilized RNA in self-collected blood using a custom tube.
  • Stabilized RNA is essential for downstream gene expression analysis.

Purpose of the Study:

  • Introduce homeRNAmax, an improved kit for remote blood RNA stabilization.
  • Enhance compatibility with commercially available blood collection tubes (BD Microtainer).
  • Increase stabilized sample volume compared to the original homeRNA kit.

Main Methods:

  • homeRNAmax kit interfaces with BD Microtainer tubes for blood collection.
  • Pilot study with 19 participants using homeRNAmax and Tasso+ device.
  • Theoretical modeling of the fluidic feature preventing stabilizer spillage.

Main Results:

  • homeRNAmax yielded high-quality RNA (mean RIN = 7.8) suitable for analysis.
  • Sufficient RNA yield (mean = 1.93 μg) was achieved in the pilot study.
  • Participants (17/19) found the homeRNAmax kit easy to use, enabling remote collection across the US.

Conclusions:

  • homeRNAmax is a user-friendly and effective tool for remote blood RNA stabilization.
  • The kit is compatible with various blood collection methods using BD Microtainer tubes.
  • Ongoing studies, including rheumatology, confirm homeRNAmax's utility in clinical research.