Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Rocky Mountain Spotted Fever01:26

Rocky Mountain Spotted Fever

Rocky Mountain Spotted Fever (RMSF) is a severe tick-borne illness caused by Rickettsia rickettsii, a Gram-negative, coccobacillary bacterium. This pathogen is an obligate intracellular parasite, requiring a host cell for replication. Transmission occurs through the bite of an infected tick. In the United States, the most important vectors are Dermacentor variabilis (American dog tick) and Dermacentor andersoni (Rocky Mountain wood tick), though other tick species may also serve as vectors.
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
The Skin Microbiota01:27

The Skin Microbiota

The human skin serves as a complex ecosystem inhabited by a diverse community of microorganisms, including bacteria, fungi, and viruses. This microbiome plays a critical role in maintaining skin health and defending against pathogenic invaders. The composition of microbial communities varies significantly across different regions of the body, influenced primarily by the local levels of moisture and sebum.Regional Variation in Skin MicrobiotaCutibacterium acnes predominantly colonizes sebaceous...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A salivary histamine-binding protein from Rhipicephalus microplus and its evaluation as an anti-tick vaccine antigen.

Veterinary parasitology·2026
Same author

Stage-dependent changes in prostaglandin E₂ in tick saliva determine its immunosuppressive effects.

Ticks and tick-borne diseases·2026
Same author

Tick saliva reprograms macrophages into immunosuppressive hubs that regulate T-cell immunity in Rhipicephalus microplus infestation.

Communications biology·2026
Same author

Human scFv antibodies selected by phage display technology exhibit distinct Zika virus binding profiles and minimal cross-reactivity with dengue virus serotype 2.

International journal of biological macromolecules·2026
Same author

m<sup>6</sup>A RNA methylation modulates Zika virus infection by regulating serine proteases in <i>Aedes albopictus</i>.

bioRxiv : the preprint server for biology·2026
Same author

Blood feeding triggers the terminal differentiation of precursor cells in tick salivary glands.

Science advances·2026
Same journal

First record of the parasite fauna of Serrasalmus geryi Jegú & Santos, 1988 (Characiformes: Serrasalmidae) and ecological implications of its invasion in a Neotropical floodplain.

Parasitology research·2026
Same journal

Molecular detection of Toxoplasma gondii in marine fish from Tunisia: First report in the Southern Mediterranean Sea.

Parasitology research·2026
Same journal

Identification and multi-omics analysis of extracellular vesicles from the plerocercoids of Spirometra mansoni (Cestoda: Diphyllobothriidae).

Parasitology research·2026
Same journal

Molecular detection and zoonotic genotyping of Enterocytozoon bieneusi in Siberian tigers (Panthera tigris altaica), Northeast China.

Parasitology research·2026
Same journal

Genotypic evidence of benzimidazole resistance and population structure insights in zoonotic Trichostrongylus colubriformis from Thailand.

Parasitology research·2026
Same journal

First molecular detection and phylogenetic characterization of Balantioides coli in lowland tapir (Tapirus terrestris).

Parasitology research·2026
See all related articles

Related Experiment Video

Updated: Jun 17, 2026

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells
12:03

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells

Published on: July 23, 2017

RmTIL, a cysteine-rich protein as a putative microbiota modulator from Rhipicephalus microplus tick.

Gabriel C A Costa1,2, Gabriel F Goulart3, Fernando A A Silva3

  • 1Departamento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil. gabriel.cerqueira@unifesp.br.

Parasitology Research
|June 16, 2026
PubMed
Summary
This summary is machine-generated.

The cattle tick

Keywords:
Rhipicephalus microplusCysteine-rich proteinMicrobiotaProtease inhibitor

More Related Videos

Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles
08:03

Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles

Published on: April 6, 2022

Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization
08:30

Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

Published on: June 1, 2018

Related Experiment Videos

Last Updated: Jun 17, 2026

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells
12:03

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells

Published on: July 23, 2017

Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles
08:03

Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles

Published on: April 6, 2022

Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization
08:30

Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

Published on: June 1, 2018

Area of Science:

  • Parasitology
  • Molecular Biology
  • Biochemistry

Background:

  • The cattle tick, Rhipicephalus microplus, causes substantial economic losses globally.
  • Understanding tick-specific proteins is crucial for developing control strategies.

Purpose of the Study:

  • To identify and characterize immunogenic proteins in Rhipicephalus microplus.
  • To investigate the function of a novel trypsin inhibitor-like (TIL) protein (RmTIL) in tick biology and reproduction.

Main Methods:

  • Phage display and third-generation sequencing (TGS) for protein identification.
  • Recombinant protein expression and functional assays for protease inhibition.
  • RNA interference (RNAi) for gene silencing and assessment of reproductive and microbial impacts.

Main Results:

  • A novel cysteine-rich protein, RmTIL, with a trypsin inhibitor-like (TIL) domain was identified.
  • Recombinant RmTIL inhibited host proteases (human neutrophil elastase, cathepsin G) and subtilisin A.
  • Gene silencing of RmTIL affected tick egg viability and altered ovarian and egg bacterial loads, impacting host-microbe interactions and embryo development pathways.

Conclusions:

  • RmTIL is an immunogenic protein essential for Rhipicephalus microplus.
  • RmTIL plays dual roles in inhibiting host proteases and regulating tick microbiota, both vital for tick survival and reproduction.