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A PCR-based assay for distinguishing between 293, 293T, and 293E cell lines
Zhilong Wang1, Wenqing Gan1, Qiuyu Chen1
1Key Laboratory of Tropical Translational Medicine of Ministry of Education, International Center for Aging and Cancer, Hainan Medical University, Haikou, 571199, China.
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Human embryonic kidney (HEK) 293 cells and their derivatives, such as 293T and 293E, are widely used in biological research and bioproduction. However, mislabeling among these sublines poses significant risks to experimental reproducibility and data interpretation. In this study, we developed a PCR-based assay to distinguish between 293, 293T, and 293E cell lines. Validation using five 293 sublines confirmed that the assay reliably identifies each line. This approach provides a simple, cost-effective tool for authenticating 293 sublines and ensuring the appropriate cell line is used in experiments.
