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Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...

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Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
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Published on: November 2, 2009

Fluorescence spectroscopy on smooth and rough solid pharmaceutical surfaces.

Rikke Helstrup1, Niels Peter Aae Christensen2, Dan Henrik Sørensen2

  • 1Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen 2100 Copenhagen, Denmark; Oral Drug Product Development, Novo Nordisk A/S, 2760 Måløv, Denmark.

International Journal of Pharmaceutics
|June 16, 2026
PubMed
Summary
This summary is machine-generated.

This study explored solid-state fluorescence spectroscopy for quantifying active pharmaceutical ingredients (API) in low-dose formulations. Results show API concentration and compaction pressure systematically affect fluorescence signals, enabling potential non-destructive analysis.

Keywords:
ChemometricsFluorescence spectroscopyLIFPATProcess analytical technologies

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Area of Science:

  • Pharmaceutical Science
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Fluorescence spectroscopy offers sensitive, fast, non-destructive analysis for pharmaceutical process analytical technology (PAT).
  • Understanding physical factors influencing fluorescence signals in solid dosage forms is crucial for accurate quantification of active pharmaceutical ingredients (API).

Purpose of the Study:

  • To systematically investigate the impact of API concentration, surface roughness, and compaction pressure on fluorescence signals in low-dose solid pharmaceutical formulations.
  • To advance the fundamental understanding of fluorescence behavior in solid pharmaceutical systems for PAT applications.

Main Methods:

  • Utilized bench-top solid-state fluorescence spectroscopy.
  • Prepared model formulations with varying tryptophan concentrations (0.10-0.50% w/w), compaction pressures, and granule milling screen sizes.
  • Analyzed fluorescence response in powder blends, compacts, and granules.

Main Results:

  • Observed distinct fluorescence signal variations with API concentration across different sample forms (powder, compact, granule).
  • Compaction pressure systematically altered fluorescence signals on both smooth and rough surfaces.
  • Granule particle size influenced fluorescence, but milling screen size did not; however, a regression model showed promising API concentration predictions.

Conclusions:

  • Fluorescence spectroscopy shows potential for non-destructive API quantification in low-dose formulations.
  • Systematic understanding of factors like API concentration and compaction pressure is key to optimizing fluorescence-based PAT.
  • Further development of fluorescence spectroscopy is supported for addressing pharmaceutical analysis challenges.