Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 18, 2026

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Published on: March 17, 2023

A Large-Scale Study Identifies a TgAb Interference Threshold for Thyroglobulin Immunoassays Using Segmented

Qianhui Liu¹, Lan You¹, Heye Lv¹

¹Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.

The Journal of Applied Laboratory Medicine

|June 16, 2026

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Prognostic Value of the TyG Index and CHG Index in Gastric Cancer.

Journal of evidence-based medicine·2026

Same author

Hyaluronic acid-based multifunctional biomaterials: Cartilage/skin regeneration, vitreous substitutes, and drug delivery systems.

Advances in colloid and interface science·2026

Same author

Surface-enhanced Raman spectroscopy for comprehensive urinalysis: advances, challenges, and clinical perspectives.

The Analyst·2026

Same author

Immunomodulatory effects of nerve stimulator-guided brachial plexus block with laryngeal mask general anesthesia in pediatric upper limb surgery.

Frontiers in pediatrics·2026

Same author

l‑Boronophenylalanine (l-BPA) Dipeptide Prodrugs Enhance Boron Delivery to Tumors, Facilitate Tumor Eradication and Induce Tumor Vaccine Effect in Mice Following Neutron Irradiation.

ACS pharmacology & translational science·2026

Same author

An interpretable machine learning tool for rheumatoid arthritis screening: integrating novel cellular morphological parameters with routine blood count indices.

Clinical rheumatology·2026

A new threshold for anti-thyroglobulin antibodies (TgAbs) near 48 U/mL on the Roche e801 platform helps accurately interpret thyroglobulin (Tg) levels in differentiated thyroid cancer (DTC) follow-up. This finding addresses interference issues in Tg immunoassays.

Area of Science:

Endocrinology
Clinical Chemistry
Oncology

Background:

Thyroglobulin (Tg) measurement is crucial for monitoring differentiated thyroid cancer (DTC).
Anti-thyroglobulin antibodies (TgAbs) can interfere with Tg immunoassays, leading to inaccurate results.
Existing TgAb cutoffs are inconsistent, necessitating an assay-specific interference threshold.

Purpose of the Study:

To establish a reliable TgAb interference threshold for Tg immunoassay interpretation on the Roche cobas e801 platform.
To validate this threshold using clinical data from papillary thyroid carcinoma (PTC) patients.

Main Methods:

Analysis of 85,949 euthyroid health-screening records using segmented linear regression to estimate a TgAb interference threshold.
External validation using 125 TgAb-positive PTC patients with receiver operating characteristic (ROC) analyses.

More Related Videos

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

Published on: July 5, 2022

Related Experiment Videos

Last Updated: Jun 18, 2026

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Published on: March 17, 2023

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

Published on: July 5, 2022

Two ROC analyses were performed comparing different patient groups based on TgAb levels and recurrence status.

Main Results:

A population-derived TgAb interference threshold of 48.1 U/mL (95% CI, 45.1-50.6) was identified.
Above this threshold, Tg values showed a tendency towards lower ranges with increased asymmetry.
ROC analyses in the PTC cohort yielded thresholds of 45.64 U/mL and 50.14 U/mL, demonstrating strong internal stability and clinical relevance.

Conclusions:

A TgAb level around 48 U/mL serves as a practical interference threshold for Tg immunoassay interpretation on the Roche cobas e801.
This threshold effectively stratifies Tg interpretability in DTC follow-up.
The established threshold aligns with ROC-derived cutoffs in TgAb-positive PTC patients, confirming its clinical utility.