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Updated: Jun 18, 2026
06:27
In Vivo Whole-Brain Imaging of Zebrafish Larvae Using Three-Dimensional Fluorescence Microscopy
Published on: April 28, 2023
Three-dimensional voltage imaging in live larval zebrafish brains using fully genetically encoded voltage indicator
Eun-Seo Cho1, Minho Eom1, Shihao Zhou2,3,4
1School of Electrical Engineering, KAIST, Daejeon, Republic of Korea.
Scientific Reports
|June 16, 2026
Summary
This study introduces a new method for in vivo three-dimensional voltage imaging in zebrafish. The technique achieves high-speed volumetric imaging, enabling better visualization of neuronal circuit dynamics.
Area of Science:
- Neuroscience
- Biophysics
- Optical Imaging
Background:
- Voltage imaging records membrane potential changes in cells with high temporal precision.
- Three-dimensional (3D) imaging is crucial for understanding complex brain circuits.
- Current 3D voltage imaging methods face challenges in speed, signal-to-noise ratio, and volume coverage.
Purpose of the Study:
- To develop and demonstrate an effective in vivo 3D voltage imaging technique for large neuronal populations.
- To overcome the limitations of existing methods for high-speed volumetric neural activity recording.
Main Methods:
- Utilized oblique plane microscopy combined with QFDBD-QUAS-driven expression of the genetically encoded voltage indicator Ace-mNeon2-Kv2.1.
- Performed in vivo 3D voltage imaging in larval zebrafish.
- Achieved high volumetric imaging rates.
Main Results:
- Demonstrated in vivo 3D voltage imaging in larval zebrafish.
- Achieved volumetric imaging rates of up to 200 volumes per second (VPS).
- Enabled dye-free voltage imaging, simplifying experimental workflows.
Conclusions:
- The developed method offers a powerful tool for high-speed, dye-free in vivo 3D voltage imaging.
- This advancement facilitates the investigation of neuronal circuit dynamics in living organisms.
- Improves reproducibility for in vivo voltage imaging experiments.
